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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Link to relevant study record(s)

Description of key information

EC50 (invertebrates)= 21 mg/l (95% CL: 12-38 mg/l), 48 hour, freshwater, OECD 202, Harlan Laboratories Ltd. 2013
NOEC (invertebrates)= 12 mg/l, 48 hour, freshwater, OECD 202, Harlan Laboratories Ltd. 2013
LOEC (invertebrates)= 38 mg/l, 48 hour, freshwater, OECD 202, Harlan Laboratories Ltd. 2013

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
21 mg/L

Additional information

The acute toxicity to Daphnia magna was carried out according to OECD 202 Daphnia sp., Acute Immobilisation Test and EU Method C.2 guidelines. A pre-study media preparation trial indicated that the use of a saturated solution method of preparation was most appropriate for this test item. Following a preliminary range-finding test, twenty daphnids (4 replicates of 5 daphnids) were exposed to an aqueous solution of the test item at nominal concentrations of 0.1 0, 0.32, 1.0, 3.2, 10, 32 and 100% v/v saturated solution for 48 hours at a temperature of approximately 20 °C under static test conditions. The test item solution was prepared by stirring an excess of test item in test medium using a propeller stirrer. After the stirring period any undissolved test item was removed by filtration to produce a 100% v/v saturated solution of the test item. The number of immobilized Daphnia were recorded after 24 and 48 hours. As the measured concentrations at 48 hours were between 93 - 118% of the measured concentrations at 0 hours, it was considered justifiable to base the results on the 0-Hour measured concentrations. The study met the acceptability criteria prescribed by the guideline. The 48h-EC50 was 21 mg/l (95% CL: 12 – 38 mg/l) based on 0-hour measured concentration.