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Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Study performed according to OECD guideline and in compliance with GLP criteria.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1996
Report date:
1996

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 A (Ready Biodegradability: DOC Die Away Test)
Qualifier:
according to guideline
Guideline:
EU Method C.4-A (Determination of the "Ready" Biodegradability - Dissolved Organic Carbon (DOC) Die-Away Test)
Qualifier:
according to guideline
Guideline:
ISO 7827 (Evaluation in an Aqueous Medium of the "Ultimate" Aerobic Biodegradability of Organic Compounds - Method by Analysis of Dissolved Organic Carbon (DOC))
GLP compliance:
yes

Test material

Constituent 1
Reference substance name:
143-30-3
IUPAC Name:
143-30-3
Details on test material:
- Name of test material (as cited in study report): 2,5-Dimethyl-3-hexin-2,5-diol
- Physical state: white solid
- Storage condition of test material: at room temperature

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic, non-adapted
Details on inoculum:
Activated sludge from in laboratory waste water treatment setting (operated with municipal and synthetic sewage sludge).
Duration of test (contact time):
29 d
Initial test substance concentrationopen allclose all
Initial conc.:
29.6 mg/L
Based on:
test mat.
Initial conc.:
20 mg/L
Based on:
DOC
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
DOC removal
Reference substance
Reference substance:
aniline

Results and discussion

Test performance:
- Degradation degree of the reference substance (% DOG): 96 after 5 days.
- Degradation degree in the inhibition control (% DOC): 45 after 14 days.
- Physico-chemnical (abiotic) elimination of the test substance (% DOC): <10 after 29 days.
- Elimination of the test substance by adsorption (% DOC): 0 after 7 days.
% Degradation
Parameter:
% degradation (DOC removal)
Value:
0
Sampling time:
29 d

Applicant's summary and conclusion

Interpretation of results:
under test conditions no biodegradation observed