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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Study performed according to OECD guideline and in compliance with GLP criteria.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1998
Report date:
1998

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
2,5-dimethylhexyne-2,5-diol
EC Number:
205-533-4
EC Name:
2,5-dimethylhexyne-2,5-diol
Cas Number:
142-30-3
Molecular formula:
C8H14O2
IUPAC Name:
2,5-dimethylhex-3-yne-2,5-diol
Details on test material:
- Name of test material (as cited in study report): 2,5-Dimethyl-3-hexin-2,5-diol
- Physical state: white homogeneous solid

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
Test substance analysis was performed for nominal concentrations of 0, 12.5 and 100 mg/L. Samples were stored in a refrigerator at 4°C until analysis.

Test solutions

Vehicle:
no
Details on test solutions:
The test substance was stirred in M4-medium for about 10 minutes at 22±1°C to obtain a stock solution of 100 mg/L (nominal). The stock solution was further diluted with water to obtain the required test substance concentrations.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Strain: STRAUS
- Source: Institut National de Recherche Chimique Appliquee, France. Cultured under standard conditions in the Laboratory of Ecotoxicology BASF AG (Ludwigshafen) since the beginning of 1978.
- Age at study initiation: 2-24 hours

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Hardness:
2.47 mmol/L
Test temperature:
20-21.2°C
pH:
7.9-8.0
Dissolved oxygen:
8.3-8.5 mg/L
Nominal and measured concentrations:
- Nominal: 0 (control), 12.5, 25, 50 and 100 mg/L
- Measured: >80% recovery.
Details on test conditions:
TEST SYSTEM
- Test vessel: 20 mL test tubes containing 10 mL test medium
- Type: open
- Aeration: yes,
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 0.5/mL

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: day:night = 16h:8h
- Light intensity: ca. 5 - 6 µE/(m²·s) at a wavelength rate of 400-700 nm

Results and discussion

Effect concentrations
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
Results are expressed as nominal concentrations as mean measured concentrations were >80% of nominal.

Applicant's summary and conclusion