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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
31 Aug 1999 to 02 Sep 1999
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
GLP compliance:
yes
Specific details on test material used for the study:
- Name of test material (as cited in study report): Deriphat 160 C KPC
- Source: Henkel KGaA, Kepec
- Water solubility: Soluble
- Volatile at room temperature: No
- Content: 30 % aqueous solution
- Batch No.: 1091821/410
- Expiration date: 30 May 2001
- Appearance: Yellow-brown / liquid
Analytical monitoring:
yes
Vehicle:
no
Details on test solutions:
PREPARATION OF TEST SOLUTION:
A clear stock solution was prepared with a target concentration of 0.5 g test substance / 250 mL M4 medium.
Test organisms (species):
Daphnia magna
Details on test organisms:
- Common name: Water flea
- Source: BioInternational B.V. NJ Horn, Netherlands
- Feed: Green algae Scenedesmus subspicatus. Organisms were fed last 21 hours before the study initiation
- Lighting: ~900 Lux; 16h:8h light:dark regime
- Temperature: ~20 °C
- Medium: same as test

EPHIPPIEN-INCUBATION
- Lighting: ~4800 Lux / continuously
- Temperature: ~20 °C
- Test medium: same as test
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Test temperature:
21 °C
pH:
7.9 - 8.2
Dissolved oxygen:
97 to 98 % (oxygen content)
Nominal and measured concentrations:
- Nominal concentrations: 0 (control), 2, 4, 8, 16, and 32 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: 100 mL glass beakers
- Type: Closed
- Fill volume: 50 mL
- No. of organisms per vessel: 10
- No. of vessels per concentration: 2
- Preparation: Correspondings aliquots of stock solution were taken while stirred continuously and pipetted into the test vessels. It was complemented with 40 mL volume of M4 medium to set the final volumes.

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Elendt M4 medium
- Culture medium different from test medium: No

OTHER TEST CONDITIONS
- Adjustment of pH:
- Photoperiod: 16 h light : 8 hours dark
- Light intensity: Approximately 900 lux

EFFECT PARAMETERS MEASURED
- Immobilisation: Immobilisation was recorded after 24 and 48 hours exposure.
Reference substance (positive control):
yes
Remarks:
Potassium dichromate
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
1.71 mg/L
Nominal / measured:
estimated
Conc. based on:
act. ingr.
Basis for effect:
mortality
Remarks on result:
other: The recovery of the test substance was approximately 60.5%. This effect value has been adjusted accordingly.
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
5.7 mg/L
Nominal / measured:
estimated
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: The recovery of the test substance was approximately 60.5%. This effect value has been adjusted accordingly.
Duration:
48 h
Dose descriptor:
LC100
Effect conc.:
19 mg/L
Nominal / measured:
estimated
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: The recovery of the test substance was approximately 60.5%. This effect value has been adjusted accordingly.
Duration:
48 h
Dose descriptor:
LC0
Effect conc.:
< 1.2 mg/L
Nominal / measured:
estimated
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: The recovery of the test substance was approximately 60.5%. This effect value has been adjusted accordingly.
Details on results:
After 48 hours, immobility was observed from the lowest test concentration and increased in a dose-reponsive manner to full immobility at the highest test concentration. Due to a low analytical substance finding (<80 %) during incubation, the effect concentrations based on nominal concentrations were adjusted. Daphnids showed no abnormal behaviour during the test period. See 'Any other information on results incl. tables'.
Results with reference substance (positive control):
The 24-h EC50 of the reference substance potassium dichromate was 1.0 mg/L, thus ranged between 0.9 and 1.9 mg/L and therefore meets the validity criteria of the standard.
Reported statistics and error estimates:
The evaluation was carried out according to the Probit method.

Table: Immobilisation results

Concentration

Replicate

Number of immobilised daphnids

After 24 h

After 48 h

0

1

0

0

2

0

0

2

1

1

1

2

0

2

4

1

0

1

2

0

4

8

1

0

4

2

0

3

16

1

2

9

2

3

9

32

1

5

10

2

7

10

Validity criteria fulfilled:
yes

Description of key information

The 48-h EC50 value is 1.71 mg a.i./L in aquatic invertebrates (Daphnia magna).

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
1.71 mg/L

Additional information

The acute toxicity to aquatic invertebrates was determined in a study according to EU Method C.2. in compliance with GLP criteria (Henkel KGaA, 1999). In this study daphnids (Daphnia magna, 20 per concentration) were exposed to nominal test concentrations of 0 (control), 2, 4, 8, 16, and 32 mg/L for 48 hours under static conditions, corresponding to concentrations of 0.6, 1.2, 1.8, 2.4, 4.8 and 9.6 mg a.i./L. Analytical confirmation of the test substances was determined by fluid chromatography with a diode-array-detector. The mean recovery rate of the test substance was found to be 60.5%. Effect values were adjusted accordingly. After 48 hours, immobility was observed from the lowest test concentration and increased in a dose-reponsive manner to full immobility at the highest test concentration. Based on the results, the 48-h EC50 was determined to be 1.71 mg a.i./L.