Dimethyl cyclohexane-1,4-dicarboxylate

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study but conducted using nominal concentrations and not performed under GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1991

Report date:

1991

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

other: EPA 660/3- 75/009, Methods for acute toxicity tests with fish, macroinvertebrates and amphibians

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

other: EPA 600/4-85/013, Methods for measuring the acute toxicity of effluents to freshwater and marine organisms, 3rd ed.

Deviations:

no

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

1,4-dimethyl cyclohexane-1,4-dicarboxylate (trans)

Cas Number:

3399-22-2

Molecular formula:

C10H16O4

IUPAC Name:

1,4-dimethyl cyclohexane-1,4-dicarboxylate (trans)

Details on test material:

- Name of test material (as cited in study report): trans-DMCD
- Lot # 910521

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Pimephales promelas

Details on test organisms:

Test specimens of Pimephales promelasoriginated from in-house cultures maintained in YMA 's aquatic toxicology laboratory. Fathead minnows used in the test were 48 days old on the day of testing.

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Hardness:

Dilution water hardness: time 0 = 120 mg/L, time 48 hours = 112 mg/L

Test temperature:

24 ± 1C

pH:

7.3-8.1

Dissolved oxygen:

1.9 - 8.1

Nominal and measured concentrations:

0, 10, 18, 32, 56, & 100 mg/L nominal only

Details on test conditions:

Each definitive test was conducted using at least five concentrations of trans-DMCD plus a dilution water control. Two replicate test vessels per treatment level were used, with each replicate holding 10 test organisms (n = 20/treatment level). The fathead minnow test was conducted in 1-L glass vessels holding 500 ml of solution. Each test was considered initiated with the completion of the distribution of test organisms to their respective test vessels. Impartial distribution was performed by sequentially adding one to two organisms to each test chamber until all test chambers had their complement of 10 organisms. Distribution was completed within 30 minutes of the preparation of exposure solutions. All test vessels were placed in an environmental chamber at 24+ 1C under 50-100 ft-candles of light and a 8-hour dark/16-hour light photoperiod. Mortality and any abnormal effects noted on the fathead minnows were recorded at 0, 24, 48, 72 and 96 hours. At the 48-hour period, new test solutions were prepared and the fish
were transferred from the old to the new solutions.

Results and discussion

Effect concentrations

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

23 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 95% CL = 21-26

Details on results:

No control mortality occurred during the 96-hour test. Concentrations of 100, 56, 32, 18 and 10 mg/L caused 100, 100, 95, 10 and 0% mortality,
respectively. No control mortality or abnormal effects existed in this test. In general, most surviving fish appeared normal with several fish in the 18 and 32 mg/L level showing a loss of equilibrium and/or air-gulping behavior.

Reported statistics and error estimates:

LC50 calculated using Trimmed Spearman-Karber method

Applicant's summary and conclusion

Executive summary:

The aquatic toxicity of trans-DMCD was determined for fathead minnows, Pimephales promelas, in a 96-hour, static renewal test conducted according to EPA 660/3-75-009, Methods for acute toxicity tests with fish, macroinvertebrates and amphibians and EPA 600/4-85/013, Methods for measuring the acute toxicity of effluents to freshwater and marine organisms, 3rd ed. The test was conducted using five nominal concentrations of trans-DMCD plus a dilution water control. Two replicate test vessels per treatment level were used, with each replicate holding 10 test organisms (n = 20/treatment level). No control mortality occurred during the 96-hour test. Concentrations of 100, 56, 32, 18 and 10 mg/L caused 100, 100, 95, 10 and 0% mortality, respectively. The 96 -hour LC50 was calculated to be 23 mg/L.