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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Eye irritation

Currently viewing:

Administrative data

Endpoint:
eye irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Comparable to Guideline study with acceptable restrictions (no GLP study, only 50 µl applied, partly limited documentation, e.g. no details about the test substance [TS] or scoring 1 h after instillation)

Data source

Referenceopen allclose all

Reference Type:
study report
Title:
Unnamed
Year:
1966
Report date:
1966
Reference Type:
study report
Title:
Unnamed
Year:
2004
Report date:
2004

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 405 (Acute Eye Irritation / Corrosion)
Deviations:
no
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Thiodiglycol
EC Number:
203-874-3
EC Name:
Thiodiglycol
Cas Number:
111-48-8
Molecular formula:
C4H10O2S
IUPAC Name:
2-[(2-hydroxyethyl)sulfanyl]ethan-1-ol
Details on test material:
no further data

Test animals / tissue source

Species:
rabbit
Strain:
Vienna White
Details on test animals or tissues and environmental conditions:
no data

Test system

Vehicle:
unchanged (no vehicle)
Controls:
other: adjacent eye treated with physiological saline
Amount / concentration applied:
undiluted
Amount applied: 0.05 ml into the conjunctival sac
Duration of treatment / exposure:
Eyes were not rinsed.
Observation period (in vivo):
Scoring:
Several times on application day and 24 hrs, 48 hrs, 72 hrs as well as 6 and 8 days after application .
Number of animals or in vitro replicates:
2

Results and discussion

In vivo

Resultsopen allclose all
Irritation parameter:
conjunctivae score
Basis:
mean
Time point:
24/48/72 h
Score:
0.83
Max. score:
1
Reversibility:
fully reversible within: 6 d
Irritation parameter:
chemosis score
Basis:
mean
Time point:
24/48/72 h
Score:
0.5
Max. score:
2
Reversibility:
fully reversible within: 6 d
Irritation parameter:
iris score
Time point:
24/48/72 h
Score:
0
Max. score:
0
Irritation parameter:
cornea opacity score
Basis:
mean
Time point:
24/48/72 h
Score:
0.5
Max. score:
1
Reversibility:
fully reversible within: 8 d
Irritant / corrosive response data:
As documented in the table below slight corneal opacity, conjunctival redness and slight or moderate conjunctival edema was found. Findings were reversible within the 3 or 8-day observation period. The control eyes which were treated with sodium chloride did not show any reactions.
Other effects:
no

Any other information on results incl. tables

Irritation Scores 

Effect redness chemosis corneal opacity iritis
rabbit No. 1 2 1 2 1 2 1 2

time after
treatment
24 h 1 1 2 0 1 0 0 0
48 h 1 1 1 0 1 0 0 0
72 h 1 0 0 0 1 0 0 0
6 days 0 nd 0 nd 1 nd 0 nd
8 days 0 nd 0 nd 0 nd 0 nd

nd: not determined

Applicant's summary and conclusion

Interpretation of results:
slightly irritating
Remarks:
Migrated information
Conclusions:
The substance caused only slight irritation to the rabbit eye after instillation of 0.05 ml thiodiglycol.
Executive summary:

Comparable to Guideline study with acceptable restrictions (no GLP study, only 50 µl applied, partly limited documentation, e.g. no details about the test substance or scoring 1 h after instillation)

Thiodiglycol (0.05 ml) was instilled into the conjunctival sac of one eye of each of 2 White Vienna rabbits (not rinsed). Effects were scored 24, 48, and 72 hours as well as 6 and 8 days after treatment. The 1st rabbit showed slight conjunctival redness (Draize score 1 at 24 - 72 hours) and moderate (Draize score 2 at 24 hours) to slight (Draize score 1 at 48 hours) conjunctival chemosis, slight corneal opacity (Draize score 1 at 24 hours up to day 6) but no iritis. All effects were reversible at day 8 (study end). In the 2nd rabbit no effects were detected except a slight redness (Draize score 1) 24 and 48 hours after instillation.

Conclusion: The substance caused only slight irritation to the rabbit eye after instillation of 0.05 ml thiodiglycol.