1-nitropropane

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1956-57 and 1981

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Non GLP, but sufficient data is available for interpetation of results.

Cross-reference

Cross-reference 1

Reason / purpose for cross-reference:

reference to same study

Cross-reference 2

Reason / purpose for cross-reference:

reference to other study

Data source

Reference

Reference 1

Reference Type:

study report

Title:

Unnamed

Year:

1957

Reference 2

Reference Type:

study report

Title:

Unnamed

Year:

1981

Reference 3

Reference Type:

other company data

Title:

Unnamed

Year:

1956

Reference 4

Reference Type:

study report

Title:

Unnamed

Year:

1956

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

Female rats were exposed to graded doses of 1-nitropropane vapor in a closed chamber for a periods of one hour. The LC50 concentrations were determined.

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

1-nitropropane

EC Number:

203-544-9

EC Name:

1-nitropropane

Cas Number:

108-03-2

Molecular formula:

C3H7NO2

IUPAC Name:

1-nitropropane

Details on test material:

1-Nitropropane :
Purity: 96.12%
Source: Sterington, Lousianna
Lot number: 9A17-7B

Test animals

Species:

rat

Strain:

Wistar

Sex:

female

Details on test animals or test system and environmental conditions:

Six female Wistar rats weighing 150 - 200 g.

Administration / exposure

Route of administration:

inhalation: vapour

Type of inhalation exposure:

whole body

Vehicle:

other: unchanged (no vehicle)

Details on inhalation exposure:

One hour inhalation exposures were conducted to determine the LD50 of the compound. The atmospheres were generated using a constant infusion pump and a vaporizer. The exposures occurred under dynamic airflow conditions.

Analytical verification of test atmosphere concentrations:

yes

Remarks:

Colormetric analysis

Duration of exposure:

1 h

Concentrations:

Actual concentrations: 8.60, 11.02, and 14.40 mg/L

No. of animals per sex per dose:

6 female rats per dose

Control animals:

not specified

Details on study design:

Female rats were exposed to graded doses of 1-nitropropane vapor in a closed chamber. One hour inhalation exposures were conducted to determine the LC50 of the compound. The atmospheres were generated using a constant infusion pump and a vaporizer. The exposures occurred under dynamic airflow conditions. Animals were observed 7 days following exposure.

Statistics:

The following endpoint was established using the Miller-Tainter method of LD50 dosage response determination.

Results and discussion

Preliminary study:

No data

Effect levels

Sex:

female

Dose descriptor:

LC50

Effect level:

11.02 mg/L air

Exp. duration:

1 h

Mortality:

Mortality observed in animals over 7 day period following exposures to 8.6, 11.02, and 14.40 mg/L where 0, 3, and 5 animals died after one hour of exposure, respectively.

Clinical signs:

other: Irritation at five minutes of exposure, no convulsant action noted except at 17 mg/L were it appeared slight. Concentration of 1-nitropropane was not sufficient for anesthetic effects. All animals exhibited ataxia following removal from the chamber. N

Body weight:

No data

Gross pathology:

No data

Other findings:

None

Any other information on results incl. tables

The acute inhalation toxicity, the LC50 was calculated from a rat LC50 at 1 hr (11.02 mg/L) by dividing by 2 as per the following guideline, "NFPA, Recommendations on the Transport of Dangerous Goods, UN Publication, 4th Ed. Section 6.4)." The calculated LC50 value of 5.5 mg/L was converted to 5500 mg/m3.

Applicant's summary and conclusion

Interpretation of results:

Toxicity Category III

Remarks:

Migrated information Criteria used for interpretation of results: other: EU GHS

Conclusions:

Following a 1 hour inhalation exposure, the LC50 is 11.02 mg/l .

Executive summary:

None