Registration Dossier
Registration Dossier
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EC number: 610-122-1 | CAS number: 433733-94-9
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 29.4.2009 - 28.5.2009
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)
- GLP compliance:
- yes
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: activated sludge freshly obtained from a municipal sewage treatment plant receiving predominantly domestic sewage
- Duration of test (contact time):
- 28 d
- Initial conc.:
- 12 mg/L
- Based on:
- other: TOC
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST SYSTEM
- Number of culture flasks/concentration: 2 replicates
CONTROL AND BLANK SYSTEM
- Inoculum blank: 2 replicates
- Positive control: 1 replicate
- Toxicity control: 1 replicate - Reference substance:
- acetic acid, sodium salt
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- > 8 - < 27
- Sampling time:
- 28 d
- Details on results:
- Mean degradation kinetic (two replicates):
Day 2: 0%
Day 14: 12%
Day 21: 14%
Day 29: 17%
The criterion for ready biodegradability (at least 60% biodegradation within a 10-day window) was not met. In the toxicity control, the test substance was found not to inhibit microbial activity. - Results with reference substance:
- Degradation kinetic:
Day 2: 15%
Day 7: 54%
Day 9: 62%
Day 14: 71% - Interpretation of results:
- not readily biodegradable
- Conclusions:
- The relative biodegradation values calculated from the measurements performed during the test period revealed 8 and 27% biodegradation of LDT600 C3, for the duplicate bottles tested. Thus, the criterion for ready biodegradability (at least 60% biodegradation within a 10-day window) was not met.
Reference
Description of key information
The relative biodegradation values calculated from the measurements performed during the test
period revealed 8 and 27% biodegradation of LDT600 C3 / DAT, for the duplicate bottles tested. Thus,
the criterion for ready biodegradability (at least 60% biodegradation within a 10-day window) was
not met.
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
Additional information
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