Guanidine, N-phenyl-, compd. with carbonic acid, hydrate (2:1:2)

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

August - October 1992

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes

Remarks:

This study has been performed in compliance with GLP in Switzerland, Procedures and Principles, March 1986, issued by the Swiss federal Department of the Interior and the Intercantonal Office for the Control of Medicaments.

Analytical monitoring:

yes

Vehicle:

no

Test organisms (species):

Daphnia magna

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

240 mg CaCO3/l

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

15.7 mg/L

Nominal / measured:

nominal

Conc. based on:

dissolved

Basis for effect:

mobility

Remarks on result:

other: 95% CL: 13.1-17.9 mg/L;

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

27.3 mg/L

Nominal / measured:

nominal

Conc. based on:

dissolved

Basis for effect:

mobility

Remarks on result:

other: 95% CL: 22.4-31.4 mg/L

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

10 mg/L

Nominal / measured:

nominal

Conc. based on:

dissolved

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

10 mg/L

Nominal / measured:

nominal

Conc. based on:

dissolved

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

32 mg/L

Nominal / measured:

nominal

Conc. based on:

dissolved

Basis for effect:

mobility

Immobilization (Initial number of daphnia = 20/test conc.)

   

Conc. nominal mg/l	Immobilization after 24 h						Immobilization after 48 h
	in vessel:				total		in vessel:				total
	1	2	3	4	number	%	1	2	3	4	number	%
Blank	0	0	0	0	0	0	0	0	0	0	0	0
5.8	0	0	0	0	0	0	0	0	0	0	0	0
10	0	0	0	0	0	0	0	0	0	0	0	0
18	0	0	0	0	0	0	5	3	3	5	16	80
32	3	5	4	5	17	85	5	5	5	5	20	100
58	5	5	5	5	20	100	5	5	5	5	20	100
100	5	5	5	5	20	100	5	5	5	5	20	100

 

Validity criteria fulfilled:

yes

Remarks:

Criteria: In the control not more than 10% of the daphnids should have been immobilised. The dissolved oxygen concentration at the end of the test should be > or equal 3 mg/l in control and test vessels.

Conclusions:

EC50,48h calculated: 15.7 mg/l
NOEC48h: 10 mg/l
EC0,48h: 10.0 mg/l
EC100,48h: 32.0 mg/l

Executive summary:

Purpose of this study was to determine the acute toxicity (EC50 (48h)) of CA 1139 A on Daphnia (Daphnia magna Straus 1820).

The test was conducted according the OECD guideline 202.

20 Daphnia were used per concentration and control. 4 replicates were conducted of 5 daphnia each.

The duration of the test was 48h. Nominal test concentrations were: 5.8, 10, 18, 32, 58 and 100 mg test substance/l.

Calculated results were as follow (values are based on nominal concentrations):

EC50,48h calculated: 15.7 mg/l

NOEC48h: 10 mg/l

EC0,48h: 10.0 mg/l

EC100,48h: 32.0 mg/l

Description of key information

Purpose of this study was to determine the acute toxicity (EC50 (48h)) of CA 1139 A on

Daphnia (Daphnia magna Straus 1820).
The test was conducted according the OECD guideline 202.
20 Daphnia were used per concentration and control. 4 replicates were conducted of 5 daphnia each.
The duration of the test was 48h. Nominal test concentrations were: 5.8, 10, 18, 32, 58 and 100 mg test substance/l.
Calculated results were as follow (values are based on nominal concentrations):
EC50,48h calculated: 15.7 mg/l
NOEC48h: 10 mg/l
EC0,48h: 10.0 mg/l
EC100,48h: 32.0 mg/l

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

15.7 mg/L

Additional information

 

Guideline Test Type	Species	Conclusion	Reference	Reliability
OECD 202	Daphnia magna (Straus 1820)	EC50(48 h) calculated: 15.7 mg/l NOEC (48 h): 10 mg/l EC0(48 h): 10 mg/l	Grade R., Ciba-Geigy, 1992	Reliable without restrictions