Guanidine, N-phenyl-, compd. with carbonic acid, hydrate (2:1:2)

Toxicity to aquatic algae and cyanobacteria

Link to relevant study record(s)

Reference

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

October 1992 - January 1993

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

Deviations:

no

GLP compliance:

yes

Remarks:

This study has been performed in compliance with GLP in Switzerland, Procedures and Principles, March 1986, issued by the Swiss federal Department of the Interior and the Intercantonal Office for the Control of Medicaments.

Analytical monitoring:

yes

Vehicle:

no

Test organisms (species):

Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Key result

Duration:

72 h

Dose descriptor:

other: EbC50 (0-72 h), calculated values

Effect conc.:

3.8 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

cell number

Remarks:

cell density

Remarks on result:

other: 95% CL: none

Duration:

72 h

Dose descriptor:

other: NOEbC (0-72 h) (5 % level)

Effect conc.:

1.1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

cell number

Remarks:

cell density

Remarks on result:

other: values observed

Inhibition of algal growth:

 

Conc. nominal mg/l	Areas under Growth Curves (A) *10000						Mean A	Inhibition * IA 0-72 h (%)
	1	2	3	4	5	6
Blank	1763	1919	2084	2004	2222	2273	2044	0.0
0.041	2213	1846	2088				2049	0.0
0.123	2495	2528	2275				2433	0.0
0.370	2488	2633	2326				2482	0.0
1.1	2108	2339	2180				2209	0.0
3.3	468	431	313				404	80.2
10	42	41	55				46	97.7

* promotion of algal growth in regard to control is indicated as 0

Validity criteria fulfilled:

yes

Remarks:

As stated in the report there were no deviations from OECD Guideline 201.

Conclusions:

EbC50,0-72h: 3.8 mg/l
NOEbC0-72h: 1.1 mg/l

Executive summary:

This study was conducted to determine the growth inhibition of CA 1139 A to Green Algae (Scenedesmus subspicatus).

The test was performed according to the OECD guideline 201.

Each test concentration was tested in 3 replicates, the blank control in 6.

Duration time of the test was 72h.

Cell densities were measured at 24, 48 and 72h exposure on a "TOA" cell counter.

Temperature was continuously measured and maintained at 23 +/- 1 °C.

pH was measured at 0 and 72h exposure.

The following results were calculated:

EbC50,0-72h: 3.8 mg/l

NOEbC0-72h: 1.1 mg/l

Values are based on nominal concentrations.

Description of key information

This study was conducted to determine the growth inhibition of CA 1139 A to Green Algae

(Scenedesmus subspicatus).
The test was performed according to the OECD guideline 201.
Each test concentration was tested in 3 replicates, the blank control in 6.
Duration time of the test was 72h.
Cell densities were measured at 24, 48 and 72h exposure on a "TOA" cell counter.
Temperature was continuously measured and maintained at 23 +/- 1 °C.
pH was measured at 0 and 72h exposure.
The following results were calculated:
EbC50,0-72h: 3.8 mg/l
NOEbC0-72h: 1.1 mg/l
Values are based on nominal concentrations.

Key value for chemical safety assessment

EC50 for freshwater algae:

3.8 mg/L

Additional information

 

Guideline Test Type	Species	Conclusion	Reference	Reliability
OECD 201	Scenedesmus subspicatus (new name: Desmodesmus subspicatus)	EbC50(0-72 h): 3.8 mg/l NOEbC (0-72 h): 1.1 mg/l	Grade R., Ciba-Geigy, 1993	Reliable without restrictions