Tetrachlorophthalic anhydride

Skin irritation / corrosion

Administrative data

Endpoint:

skin irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Study meets generally accepted scientific principles and is acceptable for assessment

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1958

Report date:

1958

Materials and methods

Principles of method if other than guideline:

BASF Test
Before OECD Guideline 404 was established in 1982, skin irritation was tested using an internal method (BASF test). White Vienna rabbits were used. Test substance was applied as an aqueous suspension (ca. 50%) to a 2.5 x 2.5 cm patch (0.5 - 1 ml) and placed on the clipped and intact skin of two white Vienna rabbits for 1 min, 5 min, 15 min and two other rabbits for 20 h under occlusive conditions. By the end of exposure, the skin was first washed with undiluted Lutrol 9, then with an aqueous Lutrol solution (50 %). Finally, cutaneous reaction was recorded daily after application of the test substance. Dermal reaction was reported in narrative form.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Tetrachlorophthalic anhydride

EC Number:

204-171-4

EC Name:

Tetrachlorophthalic anhydride

Cas Number:

117-08-8

Molecular formula:

C8Cl4O3

IUPAC Name:

tetrachloro-1,3-dihydro-2-benzofuran-1,3-dione

Details on test material:

- Name of test material (as cited in study report): Tetrachlorophthalic anhydride
- Analytical purity: no data

Test animals

Species:

rabbit

Strain:

Vienna White

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Weight at study initiation: 2.83-3.32 kg

Test system

Type of coverage:

occlusive

Preparation of test site:

not specified

Vehicle:

water

Amount / concentration applied:

0.5-1 ml of 50% aqueous Tetrachlorophthalic anhydride

Duration of treatment / exposure:

1, 5, 15 minutes and 20 hours

Observation period:

8 days

Number of animals:

2 (1, 5 and 15 minutes)
2 (20 hours)

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

erythema score

Remarks:

1, 5 & 15 min, and 20 h exposure

Basis:

mean

Remarks:

2 rabbits

Time point:

other: 24-48-72 h

Score:

0

Max. score:

4

Results 2

Irritation parameter:

edema score

Remarks:

1, 5 & 15 min, and 20 h exposure

Basis:

mean

Remarks:

2 rabbits

Time point:

other: 24-48-72 h

Score:

0

Max. score:

4

Any other information on results incl. tables

After short term exposure to the test substance for 1 to 15 minutes no skin reaction was noted. 24 hours after application of the test substance very slight erythema was observed. 

After long term exposure for 20 hours no skin reaction was noted. Three to six days after application of the test substance slight erythema was observed in one animal. No cutaneous reaction was observed on day 8 and thereafter.