Registration Dossier
Registration Dossier
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EC number: 240-362-9 | CAS number: 16251-77-7
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
Description of key information
After 72 hours the NOEC was 10 mg/L, the LOEC was 10 mg/L, the EC10 was 9.5 mg/L and the EC50 was 12 mg/L based upon growth rate.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 12 mg/L
- EC10 or NOEC for freshwater algae:
- 10 mg/L
Additional information
A GLP compliant study was performed to assess the toxicity test item to the freshwater algae Pseudokirchnerella subcapitata. The method followed was designed to be compatible with the OECD Guidelines for Testing of Chemicals (2006) No. 201, and EU Method C.3 (2009). Algae were exposed under static conditions to the substance at nominal concentrations of 1.0, 3.2, 10, 32, or 100 mg/L. For each concentration, the cell densities were determined at 0, 24, 48, and 72 hours. The test concentration was confirmed at 72-hours by HPLC analysis using additional test samples that were prepared at the start of the test with the omission of algal cells and incubated under test conditions.
The test met the validity criteria for OECD Guideline 201 as the biomass concentration in the control cultures increased by a factor of 164 after 72 hours, the mean coefficient of variation for section-by-section specific growth rates in the control cultures was 16%, and the coefficient of variation of average specific growth rates control cultures over the test period (0 – 72h) was 3%. After 72 hours the NOEC was 10 mg/L, the LOEC was 32 mg/L, the EC10 was 9.5 mg/L and the EC50 was 12 mg/L based upon growth rate; analytical confirmation indicated that the measured test concentrations were within the range of 86% to 96% of nominal.
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