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Neurotoxicity

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Description of key information

Acute and chronic administration of 3-pentenenitrile did not induce behavioral abnormalities.

Key value for chemical safety assessment

Additional information

2 publications were available on the neurotoxicity of 3-pentenenitrile:

- In a subchronic neurotoxicity study (Gagnaire et al., 1998), 12 male Sprague-Dawley rats were exposed to trans-3-pentenenitrile (96%), orally, in a volume of 2 mL/kg bw, 5 days per week, for 12 weeks at dose levels of 25, 50 and 100 mg/kg bw/day. Trans-3-pentenenitrile was dissolved in olive oil. Control groups of 10 rats received an equivalent volume of oil alone. No guideline was followed, there were no GLP indications. No deaths occured. A deficit in body weight was observed, which reached 15 % in the high dose group at the end of the exposure. No deficits were observed in any parameter studied in any treated group. Chronic administration of trans-3-pentenenitrile to male rats did not cause neurological symptoms and electrophysiological deficits in peripheral nerves.

 - In an acute neurotoxicity study (Tanii et al., 1989), male ddY mice were exposed to 3 -pentenenitrile (purity unknown). Mice were pretreated intraperitoneally either with 0.16 mL of olive oil or with 0.16 mL of a 20% CCl4 solution in olive oil per 25 g body weight, according to Tanii and Hashimoto (1984a). Twenty four hours later, animals were given orally 3-pentenitrile or olive oil in a volume of 0.1 mL per 25 g bw. Doses ranged from 0.11 to 0.36 mmol/kg for olive-oil pretreated animals (8.9 to 29.2 mg/kg bw/d) and from 0.25 to 0.81 mmol/kg for CCl4-pretreated animals (20.3 to 65.7 mg/kg bw/d). The dose levels in CCl4 -pretreated mice were higher than that in olive oil-pretreated animals because CCl4 reduced the toxicity of nitriles. Observation of behavior was done daily for 45 days. No quantitative measurement was done for the excitment and circling. After treatment with 3 -pentenenitrile, 6 and 8 animals, respectively in olive oil and CCl4 pretreated mice, died. No behavioral abnormalities were observed.

Justification for classification or non-classification

According to these observation, 3 -pentenitrile is not classified for neurotoxicity according to the CLP Regulation 1272/2008/EC and the Directive 67/548/EC.