Registration Dossier

Diss Factsheets

Administrative data

Description of key information

Oral: LD50 Males: 46.6 mg/kg bw for rat
Dermal: LD50 Males: 259.4 mg/kg bw for rat
Inhalation: LC50 Males: 1.39 mg/L for rat

Key value for chemical safety assessment

Acute toxicity: via oral route

Endpoint conclusion
Dose descriptor:
LD50
Value:
46.6 mg/kg bw

Acute toxicity: via inhalation route

Endpoint conclusion
Dose descriptor:
LC50
Value:
1 390 mg/m³

Acute toxicity: via dermal route

Endpoint conclusion
Dose descriptor:
LD50
Value:
259.4 mg/kg bw

Additional information

Acute toxicity: oral

A study was identified as the key study (Rondot, 1981). 3-Pentenenitrile was tested for acute oral toxicity similarly to OECD test guideline No. 401. Groups of Sprague-Dawley rat (5/sex) were administered by gavage a single oral dose of trans-pentene-3 nitrile (purity unknown) in aqueous suspension of 10% arabic gum at doses of  0, 60, 80, 100 or 120 mg/kg bw and observed for 14 days. Reduced activity, apathy, ptosis were observed during the study. Haemorrhagic digestive tract, thymus and lungs were noted in the animals died during the study. Oral LD50Combined = 72 mg/kg bw (58-88 mg/kg bw), LD50 males = 46.6 mg/kg bw and LD50 females = 93.9 mg/kg bw. The study showed that males are more sensitive than females.

  

Acute toxicity: dermal

A study was identified as the key study (Rondot, 1981). 3-Pentenenitrile was tested for acute dermal toxicity similarly to OECD test guideline No. 402. Groups of Sprague-Dawley rats (5/sex)were exposed to trans-pentene-3 nitrile (purity unknown) in an aqueous dispersion of arabic gum 10% for 24 hours at doses of 0, 400, 600, 800 and 1000 mg/kg bw. Animals then were observed for 14 days. Reduced activity, apathy, ptosis were observed during the study. Haemorrhagic lungs, congestioned derma and thickening of the peritoneum were noted in the animals died during the study. Dermal LD50Combined = 369 mg/kg bw (264-515 mg/kg bw), LD50 males = 259.4 mg/kg bw and LD50 females = 524.6 mg/kg bw. This study showed that males are more sensitive than females.

 

Acute toxicity: inhalation

A study was identified as the key study (Japp, 1970). In this acute inhalation toxicity study, groups of ChR-CD rats (6 males) were exposed to 338, 359, 447, 538 and 692 ppm of 3-Pentene nitrile vapors in a 16-liter bell jar for 4 hours. Gross and histopathologic examinations were performed on 2 rats eachat 1,2, 7, and 14 days post- exposure. Tissues examined included lungs, liver, spleen, kidney, testes, and thymus. The other survivors were sacrificed 14 days post-exposure. Inhalation LC50Males = 1.391 mg/L (420 ppm) (1.2 -1.58 mg/L). At lethal concentration, irregular respiration, hyperemia, red discharge around eyes, tremors, salivation, pale ears and hypersensitivity were observed during and after exposure. Death occurred from 2.5 hours to overnight. At non-lethal concentration, irregular respiration, incoordination, red discharge from nose, hindleg tremors and incontinence were observed.

Justification for classification or non-classification

Based on the LD50 males of 46.6 mg/kg bw, 3-Pentenenitrile is classified for acute oral toxicity as:

- Category 2 (H300, Fatal if swallowed) according to the CLP Regulation (1272/2008) as LD50 was found to be between 5 and 50 mg/kg bw.

- Toxic if swallowed (T; R25) according to the Directive 67/548/EC as LD50 was found to be betwwen 25 and 200 mg/kg bw.

Based on the LD50 males of 259.4 mg/kg bw, 3-Pentenenitrile is classified for acute dermal toxicity as:

- Category 3 (H311, Toxic in contact with skin) according to the CLP Regulation (1272/2008) as LD50 was found to be between 200 and 1000 mg/kg bw.

- Toxic in contact with skin (T; R24) according to the Directive 67/548/EC as LD50 was found to be betwwen 50 and 400 mg/kg bw.

Based on the LC50 males of 1.39 mg/L, 3-Pentenenitrile is classified for acute inhalation toxicity as:

- Category 2 (H330 Fatal if inhaled) according to the CLP Regulation (1272/2008) as LD50 was found to be between 0.5 and 2 mg/L/4h.

- Toxic by inhalation (T; R23) according to the Directive 67/548/EC as LC50 was found to be betwwen 0.5 and 2 mg/L/4h.