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Administrative data

Endpoint:
acute toxicity: inhalation
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1970
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Well conducted study although some information are missing.
Cross-referenceopen allclose all
Reason / purpose for cross-reference:
reference to same study
Reason / purpose for cross-reference:
reference to other study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1970
Report date:
1970

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
Six ChR-cd male rats were exposed to the test material in a 16 L bell jar for 4 hours. The test substance was metered at a uniform rate into a heated
stainless steel T-tube by a syringe drive and vaporized under prepurified nitrogen. The test material vapors were mixed with oxygen and carried into the bell jar. Houseline air was used as diluent to give the desired atmospheric concentration. Gross and histopathologic examinations were performedon two rats each at 1, 2, 7 and 14 days post exposure. The other survivors were sacrified 14 days post-exposure.
GLP compliance:
no
Test type:
standard acute method
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Pent-3-enenitrile
EC Number:
225-060-7
EC Name:
Pent-3-enenitrile
Cas Number:
4635-87-4
Molecular formula:
C5H7N
IUPAC Name:
(3E)-pent-3-enenitrile
Details on test material:
- Name of test material (as cited in study report): 3-Pentene nitrile

Test animals

Species:
rat
Strain:
other: ChR-CD
Sex:
male
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Source: no data
- Age at study initiation: no data
- Weight at study initiation: 250-289 g
- Fasting period before study: no data
- Housing: no data
- Diet (e.g. ad libitum): no data
- Water (e.g. ad libitum): no data
- Acclimation period: no data


ENVIRONMENTAL CONDITIONS
no data


IN-LIFE DATES: no data

Administration / exposure

Route of administration:
inhalation: vapour
Type of inhalation exposure:
whole body
Vehicle:
other: unchanged (no vehicle)
Details on inhalation exposure:
Male rats were exposed to the test material in a 16 L bell jar for 4 hours. The test substance was metered at a uniform rate into a heated stainless steel T-tube by a syringe drive and vaporized under prepurified nitrogen. The test material vapors were mixed with oxygen and carried into the bell jar.
Houseline air was used as diluent to give the desired atmospheric concentration.
Analytical verification of test atmosphere concentrations:
yes
Remarks:
Gas samples were taken periodically from the chamber exit and analyzed by gas chromatography.
Duration of exposure:
4 h
Concentrations:
338, 359, 447, 538, and 692 ppm
No. of animals per sex per dose:
6 animals per dose
Control animals:
not specified
Details on study design:
Gross and histopathologic examinations were performed on 2 rats each at 1, 2, 7, and 14 days post- exposure. Tissues examined included lungs, liver, spleen, kidney, testes, and thymus. The other survivors were sacrificed 14 days post-exposure.
Statistics:
Litchfield and Wilcoxin (1949)

Results and discussion

Preliminary study:
Not applicable
Effect levels
Sex:
male
Dose descriptor:
LC50
Effect level:
420 ppm
Based on:
test mat.
95% CL:
>= 362 - <= 478
Exp. duration:
4 h
Remarks on result:
other: 1.391 mg/L air (1.2-1.58)
Mortality:
No data.
Clinical signs:
other: At lethal concentration: irregular respiration, hyperemia, red discharge around eyes, tremors, salivation, pale ears and first death within 150 min were observed during exposure. Death overnight and hypersensitivity were noted after exposure. At non-leth
Body weight:
Initial weight loss followed by normal weight gain.
Gross pathology:
No anatomical evidence of primary injury.
Other findings:
No data

Any other information on results incl. tables

It was apparent that inhalation of 3 -Pentene nitrile exerted some sort of stress on the animals, but the nature of this effect was not revealed by microscopic examination of the tissues that were examined.

Applicant's summary and conclusion

Interpretation of results:
Toxicity Category II
Remarks:
Migrated information Criteria used for interpretation of results: EU
Conclusions:
Under the test conditions, 3-Pentene Nitrile is classified in Category 2 (H330: fatal if inhaled) according to the CLP Regulation 1272/2008/EC and as toxic by inhalation (T; R23) according to the Directive 67/548/EEC.
Executive summary:

In an acute inhalation toxicity study, groups of ChR-CD rats (6 males) were exposed to 338, 359, 447, 538 and 692 ppm of 3-Pentene nitrile vapors in a 16-liter bell jar for 4 hours.Gross and histopathologic examinations were performed on 2 rats eachat 1,2, 7, and 14 days post- exposure. Tissues examined included lungs, liver, spleen, kidney, testes, and thymus.The other survivors were sacrificed 14 days post-exposure

 

 LC50Males = 1.391 mg/L (420 ppm) (1.2 -1.58 mg/L)

 

At lethal concentration, irregular respiration, hyperemia, red discharge around eyes, tremors, salivation, pale ears and hypersensitivity were observed during and after exposure. Death occurred from 2.5 hours to overnight.

At non-lethal concentration, irregular respiration, incoordination, red discharge from nose, hindleg tremors and incontinence were observed during and after exposure.

 Under the test conditions, 3-Pentene Nitrile is classified in Category 2 (H330: Fatal if inhaled) according to the CLP Regulation 1272/2008/EC and as toxic by inhalation (T; R23) according to the Directive 67/548/EEC.