Pent-3-enenitrile

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2019-2020

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2020

Report date:

2020

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 403 (Acute Inhalation Toxicity)

Version / remarks:

September 2009

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

EPA OPPTS 870.1300 (Acute inhalation toxicity)

Version / remarks:

August 1998

Deviations:

no

Test guideline 3

Qualifier:

according to guideline

Guideline:

other: EC No 440/2008, part B. Acute Toxicity (inhalation), May 2008, amended by COMMISSION REGULATION (EU) No 260/2014

Version / remarks:

24 January 2014

Deviations:

no

Test guideline 4

Qualifier:

according to guideline

Guideline:

other: Appendix to Director General Notification, No. 12-Nousan-8147. Agricultural Production Bureau, Ministry of Agriculture, Forestry and Fisheries of Japan (JMAFF)

Version / remarks:

November 2000

Deviations:

no

GLP compliance:

yes

Test type:

fixed concentration procedure

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Pent-3-enenitrile

EC Number:

225-060-7

EC Name:

Pent-3-enenitrile

Cas Number:

4635-87-4

Molecular formula:

C5H7N

IUPAC Name:

(3E)-pent-3-enenitrile

Details on test material:

- Name of test material (as cited in study report): 3-Pentenenitrile
- Analytical purity: > 95 %

Test animals

Species:

rat

Strain:

other: Wistar Han

Sex:

male/female

Administration / exposure

Route of administration:

inhalation: vapour

Type of inhalation exposure:

nose only

Vehicle:

air

Details on inhalation exposure:

4.9.2. Test Atmosphere Concentration Sampling
A total of 6 and 8 representative samples were taken for determination of the actual
concentration during exposure at 2 and 0.5 mg/L, respectively. Samples were drawn from the
test atmosphere through a tube mounted in one of the free animal ports of the exposure
chamber. Sample volumes were measured by means of a dry gas meter (type G 1.6, Actaris
Meterfabriek B.V., Dordrecht, The Netherlands). The samples were drawn through series of
four impingers (2 mg/L) and four or two impingers (0.5 mg/L) filled with acetonitrile as
collection fluid. The amount of test item collected was measured analytically (analytical
method developed under test facility project number 20184667). Subsequently the timeweighted mean concentrations with the standard deviations were calculated.
At 2 mg/L, only the first two impinger samples were used for calculation as from sample 3
onwards the third and fourth impingers showed high values. Normally, these impinger
samples should be negligibly small and it was concluded that a mistake was made at sample 3
which contaminated the impingers at the subsequent measurements. Sample 3 was excluded
as the concentration was unrealistically high as no changes were made for the generation
settings. Inclusion of the sample would lead to an actual concentration exceeding the amount
of test item used (nominal concentration).

Analytical verification of test atmosphere concentrations:

no

Duration of exposure:

4 h

Concentrations:

Initially, 3-PENTENENITRILE was administered as a vapor by nose only inhalation for
4 hours to one group of five male and five female Wistar Han rats at a target concentration of
2 mg/L. Based on the results, the males were identified as most sensitive sex and therefore
five males were exposed at a target concentration of 0.5 mg/L.

No. of animals per sex per dose:

2 mg/L: 5 male and 5 female rats
0.5 mg/L: 5 male rats

Control animals:

no

Results and discussion

Effect levels

Key result

Sex:

male

Dose descriptor:

LC50

Effect level:

> 0.5 - <= 2 mg/L air (nominal)

Based on:

test mat.

Exp. duration:

4 h

Mortality:

males 0.5 mg/L: 0
males 2.0 mg/L: 4
females 2.0 mg/L: 1

Body weight:

No abnormalities

Gross pathology:

At 2 mg/L, macroscopic post mortem examination revealed no test item related abnormalities
in any of the animals. At 0.5 mg/L, macroscopic examination revealed isolated black/brown
foci in the lungs of one male.