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Administrative data

Description of key information

- Skin irritation: not irritating
- Eye irritation: slightly irritating but not classify.

Key value for chemical safety assessment

Skin irritation / corrosion

Endpoint conclusion
Endpoint conclusion:
no adverse effect observed (not irritating)

Eye irritation

Endpoint conclusion
Endpoint conclusion:
adverse effect observed (irritating)

Additional information

Skin irritation/corrosion

No key study was identified for this endpoint. In the study performed by Gonnet and Guillot (1981), all the rabbits died on day 1 of the experiment, therefore, no scores could be calculated.

In the study of Franck (1968) (reliability: 4), only short abstract is available and the purity of the test substance is not stated. Despite missing information, this study showed that 3 -PN is not irritating for skin in Guinea pig. Only one guinea pig on 10 showed mild erythema, one day after application of undiluted 3 -PN. In the other tested group (3 -PN solution at 43%), no skin reactions were observed.

Moreover, no signs of irritation were observed in male and female rats in the dermal acute toxicity study (Rondot, 1981).

Taken into account the overall data, skin irritation is not expected for 3 -PN.


Eye irritation

A key study was identified (Gonnet and Guillot, 1981). In an eye irritation study performed similarly to the OECD test guideline No. 405, 0.1 mL of undiluted trans-pentene-3-nitrile was instilled into the conjunctival sac of left eye of 4 New Zealand White rabbits. The eyes of the animals were not washed after instillation. Eyes were examined at 1, 24, 48, 72, 96 and 168 hours after dosing and grading according to Draize scale. Trans-pentene-3-nitrile produced chemosis (mean score 0.42), conjunctival redness (mean score 0.25), iris lesions (mean score 0.08). Effects were completely reversed after 2 to 4 days.

Effects on eye irritation: slightly irritating

Justification for classification or non-classification

As no signs of skin irritation were observed in dermal irritation study and acute dermal study, 3-pentenenitrile is not considered as irritating to the skin according to the Directive 67/548/EC and the CLP Regulation (1272/2008).

As a slight eye irritation was observed in an eye irritation study with 3-pentenenitrile conducted similarly to the OECD test guideline No. 405, 3-pentenenitrile is considered as slightly irritating to eyes but no classification is required according to the Directive 67/548/EC and the CLP Regulation (1272/2008).