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Administrative data

Endpoint:
short-term repeated dose toxicity: inhalation
Type of information:
experimental study
Adequacy of study:
other information
Study period:
1970
Reliability:
3 (not reliable)
Rationale for reliability incl. deficiencies:
other: Documentation insufficient for assessment, no details provided. Only one dose of 3-PN was tested.
Cross-referenceopen allclose all
Reason / purpose for cross-reference:
reference to same study
Reason / purpose for cross-reference:
reference to other study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1970
Report date:
1970

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
The subacute exposure procedure was the same than described in (Acute Inh. tox. V2 1970 JAPP). Six rats were exposed for four hours each day for
two weeks (total of ten exposures). Control rats were exposed to oxygen and nitrogen for the same amount of time. Three control and three test rats were sacrified after the tenth exposure; the remaining three control and three rats were sacrificed following a 14-day recovery period.
GLP compliance:
no
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Pent-3-enenitrile
EC Number:
225-060-7
EC Name:
Pent-3-enenitrile
Cas Number:
4635-87-4
Molecular formula:
C5H7N
IUPAC Name:
(3E)-pent-3-enenitrile
Details on test material:
- Name of test material (as cited in study report): 3-Pentene nitrile

Test animals

Species:
rat
Strain:
other: ChR-CD
Sex:
male
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Source: no data
- Age at study initiation: no data
- Weight at study initiation: 250-289 g
- Fasting period before study: no data
- Housing: no data
- Diet (e.g. ad libitum): no data
- Water (e.g. ad libitum): no data
- Acclimation period: no data


ENVIRONMENTAL CONDITIONS
no data


IN-LIFE DATES: no data

Administration / exposure

Route of administration:
inhalation: vapour
Type of inhalation exposure:
whole body
Vehicle:
other: unchanged (no vehicle)
Remarks on MMAD:
MMAD / GSD: No data
Details on inhalation exposure:
Male rats were exposed to the test material in a 16 L bell jar for 4 hours. The test substance was metered at a uniform rate into a heated stainless steel T-tube by a syringe drive and vaporized under prepurified nitrogen. The test material vapors were mixed with oxygen and carried into the bell jar.
Houseline air was used as diluent to give the desired atmospheric concentration.
Analytical verification of doses or concentrations:
yes
Details on analytical verification of doses or concentrations:
Gas samples were taken periodically from the chamber exit and analyzed by gas chromatography.
Duration of treatment / exposure:
2 weeks
Frequency of treatment:
4 hours/day (total of 10 exposures)
Doses / concentrations
Remarks:
Doses / Concentrations:
55 ppm (0.18 mg/L)
Basis:
analytical conc.
No. of animals per sex per dose:
6 males/doses
Control animals:
other: Other: control rats were exposed to oxygen and nitrogen.
Details on study design:
No data
Positive control:
No

Examinations

Observations and examinations performed and frequency:
Gross and histopathologic examinations were performed on 3 control and 3 test rats after the tenth exposure.
Tissues examined included lungs, liver, spleen, kidney, testes, and thymus.
Sacrifice and pathology:
The remaining 3 control and 3 test rats were sacrificed following a 14-day recovery period.
Other examinations:
No data
Statistics:
No

Results and discussion

Results of examinations

Clinical signs:
no effects observed
Mortality:
no mortality observed
Body weight and weight changes:
no effects observed
Food consumption and compound intake (if feeding study):
not examined
Food efficiency:
not examined
Water consumption and compound intake (if drinking water study):
not examined
Ophthalmological findings:
not examined
Haematological findings:
not examined
Clinical biochemistry findings:
not examined
Urinalysis findings:
not examined
Behaviour (functional findings):
not examined
Organ weight findings including organ / body weight ratios:
not examined
Gross pathological findings:
no effects observed
Histopathological findings: non-neoplastic:
no effects observed
Histopathological findings: neoplastic:
no effects observed
Details on results:
Mild hyperemia and red discharge around eyes were observed during exposure.
Gross and histopatologic examinations showed no evidence of primary injury.

Target system / organ toxicity

Critical effects observed:
not specified

Any other information on results incl. tables

No remarks

Applicant's summary and conclusion

Conclusions:
Only mild hyperemia and red discharge around eyes were observed during exposure at the only dose tested (55 ppm). Exposure did not give any clinical or pathological indication of accumulation in exposed rats. Gross and histopatologic examinations showed no evidence of primary injury.
Executive summary:

In a subacute inhalation toxicity study, 3-Pentene nitrile was administered to male ChR-CD rats (6/dose) by dynamic whole body exposure at concentrations of 0 and 0.18 mg/L (55 ppm) for 4 hours per day, 6 days/week for a total of 10 exposures. 3PN was metered at a uniform rate into a stainless steel T-tube by a syringe drive and vaporized under prepurified nitrogen in a 16-liter bell jar.

Gross and histopathologic examinations were performed on 3 control and 3 test rats after the tenth exposure. Tissues examined included lungs, liver, spleen, kidney, testes, and thymus. The remaining 3 control and 3 test rats were sacrificed following a 14 -day recovery period.

 

Only mild hyperemia and red discharge around eyes were observed during exposure at 55 ppm (0.18 mg/L). Exposure did not give any clinical or pathological indication of accumulation in exposed rats. Gross and histopatologic examinations showed no evidence of primary injury.