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Bioaccumulation: aquatic / sediment

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Endpoint:
bioaccumulation in aquatic species: fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Bioaccumulation test in fish performed by MITI.
Qualifier:
according to guideline
Guideline:
other: Bioaccumulation test of a chemical substance in fish or shellfish by MITI
GLP compliance:
not specified
Radiolabelling:
not specified
Test organisms (species):
Cyprinus carpio
Total exposure / uptake duration:
6 wk
Details on test conditions:
RANGE-FINDING / PRELIMINARY STUDY : TLm(48h) > 80 mg/L, Rice fish (Oryzias latipes)
Lipid content:
3.5 %
Type:
BCF
Value:
24 - 49 L/kg
Remarks on result:
other: Conc.in environment / dose:0.8 mg/L
Type:
BCF
Value:
72 - 184 L/kg
Remarks on result:
other: Conc.in environment / dose:0.08 mg/L
Validity criteria fulfilled:
not specified
Conclusions:
The biocconcentration factor (BCF) was determined to be 24-49 L/Kg at an enviornmental concentration of 0.8 mg/L, and 72-184 L/Kg at an environmental concentration of 0.08 mg/L in carp fish (Cyprinus carpio) after 6 week exposure period.
Executive summary:

A bioaccumulation test in fish was performed by MITI. A range finding study was performed on Rice fish (Oryzias latipes) were LC50 after 48 hours exposure was > 80 mg/L. In the main test, carp fish (Cyprinus carpio) were exposed to 0.8 (1st concentration area) and 0.08 mg/L (2nd concentration area) test substance during 6 weeks. The lipid content was 3.5%. The biocconcentration factor (BCF) was determined to be 24-49 L/Kg at an enviornmental concentration of 0.8 mg/L, and 72-184 L/Kg at an environmental concentration of 0.08 mg/L.

Endpoint:
bioaccumulation: aquatic / sediment
Type of information:
calculation (if not (Q)SAR)
Remarks:
Estimated by calculation
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Internationally accepted method, EPI Suite, EPA (USA)
Principles of method if other than guideline:
EPI Suite BCFBAF v3.01
GLP compliance:
no
Test type:
other: estimated by calculation
Details on estimation of bioconcentration:
BASIS FOR CALCULATION OF BCF
- Estimation software: EPI Suite BCFBAF v3.01
- Result based on calculated log Pow of: 9.78
Key result
Type:
BCF
Value:
576 L/kg
Calculation basis:
other: EPI Suite BCFBAF v3.01

Bioaccumulation Estimates (BCFBAF v3.01):

Log BCF from regression-based method = 2.760 (BCF = 576 L/kg wet-wt)

Log Biotransformation Half-life (HL) = -1.7554 days (HL = 0.01756 days)

Log BCF Arnot-Gobas method (upper trophic) = -0.045 (BCF = 0.9021)

Log BAF Arnot-Gobas method (upper trophic) = -0.045 (BAF = 0.9021)

log Kow used: 9.78 (estimated)

Validity criteria fulfilled:
not applicable
Conclusions:
BCF EPISUITE = 576 L/kg wet-wt (BCFBAF v3.01)
Executive summary:

BCF EPISUITE = 576 L/kg wet-wt (BCFBAF v3.01)

Description of key information

Key study: Experimental data. Test performed by MITI. The biocconcentration factor (BCF) was determined to be 24-49 L/Kg at an enviornmental concentration of 0.8 mg/L and 72-184  L/Kg at an environmental concentration of 0.08 mg/L in carp fish (Cyprinus carpio).

Key value for chemical safety assessment

BCF (aquatic species):
184 L/kg ww

Additional information

Key study: Experimental data:

A bioaccumulation test in fish was performed by MITI. Carp fish (Cyprinus carpio) were exposed to 0.8 and 0.08 mg/L test substance during 6 weeks. The lipid content was 3.5%. The biocconcentration factor (BCF) was determined to be 24-49 L/Kg at an enviornmental concentration of 0.8 mg/L, and 72-184 L/Kg at an environmental concentration of 0.08 mg/L in carp fish (Cyprinus carpio) after 6 week exposure period.

Supporting study:

Estimated value for the main component of the substance:

Main component 1: BCF EPISUITE = 576 L/kg wet-wt (BCFBAF v3.01)

The main component of this multiconstituent substance have an extreme log Pow and a high molecular weight and considered as not bioavailable. The components are unlikely to cross biological membranes. Applying Lipinski´s Rule of Five, the substance is not bioavailable and therefore, there is no potential for bioaccumulation.