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EC number: 267-022-2 | CAS number: 67762-53-2
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
Description of key information
EC50 (3 h) > 1000 mg/L (OECD 209)
Key value for chemical safety assessment
Additional information
Since no studies investigating the toxicity to aquatic algae and cyanobacteria offatty acids, C5-9 tetraesters with pentaerythritol (CAS 67762-53-2) are available for this endpoint, in accordance to Regulation (EC) No. 1907/2006 Annex XI, 1.5 a read across to three structurally related category membersdecanoic acid, mixed esters with heptanoic acid, octanoic acid, pentaerythritol and valeric acid(71010-76-9), pentaerytritol tetraoleate (CAS 19321-40-5) and decanoic acid, ester with 2-ethyl-2-(hydroxymethyl)-1,3-propanediol octanoate (CAS 11138-60-6) was conducted. This read-across is justified in detail in the overall summary (IUCLID chapter 6.1) and within the category justification in IUCLID Section 13. In this case of read-across, the best suited (highest degree of structural similarity, nearest physico-chemical properties) read-across substances were used for the assessment. The fatty acid chain length of C5 – C10 of the first read-across substances are similar to those of the target substance and both substances contain the same alcohol (pentaerythritol) and are tetra-esters. The other two substances are covering the variability of the PE esters group within the polyol category with different alcohol components or longer fatty acid chain lengths (≥ C16).
The first study with the read-across substance decanoic acid, mixed esters with heptanoic acid, octanoic acid, pentaerythritol and valeric acid (CAS No. 71010-76-9) was conducted under static conditions according to OECD 209 investigating aquatic microorganisms (Dickinson, 2008). In this GLP studynon-adapted activated sludge obtained from a domestic sewage treatment plant was used as inoculum. Test concentrations of 10, 100 and 1000 mg/L were prepared. Test substance was added directly to the test vessels. The test substance did not inhibit the respiration rate of activated sludge microorganisms at any of the concentrations employed in the test. Hence, the 3 h-EC50 was determined to be > 1000 mg/L based on the nominal test concentration. Therefore it can be concluded that the test substance will not exhibit effects on the respiration rate of microorganisms.
The second study with the read-across substancepentaerytritol tetraoleate (CAS 19321-40-5)(CAS No. 19321-40-5) was conducted under static conditions according to ISO 10712 investigating aquatic microorganisms (Mead, 1997). In this GLP study an aqueous suspension of Pseudomonas putida was used as inoculum. A nominal test concentration of 10000 mg/L was used (prepared as WAF). The test substance did not inhibit the growth of Pseudomonas putida. Hence, the 16 h-EL50 was determined to be > 10000 mg/L based on the nominal test concentration. This outcome is confirmed by the result of the toxicity control of the biodegradation study with the same read-across substance (Desmares-Koopmans, 1997). In this study according to OECD 301B a 14 d-NOEC of 15.6 mg/L could be determined, based on the inhibition control.
The study with the third read-across substance decanoic acid, ester with 2-ethyl-2-(hydroxymethyl)-1,3-propanediol octanoate (CAS 11138-60-6) was conducted under static conditions according to OECD 209 investigating aquatic microorganisms (Kuttler, 1998). In this GLP study activated sludge obtained from a domestic sewage treatment plant was used as inoculum. A test concentration of 10000 mg/L was prepared and was added directly to the test vessels. The test substance did not inhibit the respiration rate of activated sludge microorganisms, the 3 h-EC50 was therefore determined to be > 10000 mg/L based on the nominal test concentration. Therefore it can be concluded that the test substance will not exhibit effects on the respiration rate of microorganisms.
Based on these results from structurally related read-across substances (in accordance to Regulation (EC) No 1907/2006 Annex XI, 1.5) which are characterized by a similar ecotoxicological profile, it can be concluded that fatty acids, C5-9 tetraesters with pentaerythritol (CAS 67762-53-2) will not effects on the respiration rate of microorganisms. . As it can be seen in the data matrixof the category justification in section 13 and the overall endpoint summary IUCLID 6.1, all reliable data in the polyol esters category support this hazard assessment by showing a consistent pattern of results, i.e. no microbial inhibition and no toxic effects were observed to aquatic organisms up to the limit of water solubility.
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