Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Currently viewing:

Administrative data

Link to relevant study record(s)

Description of key information

72 h ErC10: 4.7 mg/L
72 h ErC50: > 93 mg/L

Key value for chemical safety assessment

EC50 for freshwater algae:
93 mg/L
EC10 or NOEC for freshwater algae:
4.7 mg/L

Additional information

In the Klimisch 1 GLP study from Kimmel (2013) the toxicity of Butanedioic acid, sulfo-, 4-[2-[(2-hydroxyethyl)amino]ethyl] ester, N-C18-unsatd. acyl derivs., disodium salts to Desmodesmus subspicatus was determined in a static 72-Hour Algal Growth Inhibition Test. The test was performed according to OECD 201 and EU Method C.3. The nominal concentrations of 1.0, 3.2, 10, 32 and 100 mg test item/L. were tested in parallel with a control. Dose verification analysis was performed except for the nominal 1.0 mg/L test groups since this concentration was below the NOEC. The measured concentrations of the test item in the test media of the test concentrations of 3.2 to 100 mg test item/L were between 109 to 113% of the nominal values at the start of the test and between 78 to 90% at the end of the test. Thus, the correct dosing of the test item was confirmed. During the test period of 72 hours, a decrease of test item concentration in the test media occurred. The biological results were related to the mean measured test item concentrations (calculated as the geometric means of the concentrations measured at the start and end of the test), i.e., mean measured 3.2, 9.6, 30 and 93 mg/L for nominal concentrations of 3.2, 10, 32 and 100 mg test item/L, respectively. In order to determine the growth of the cultures, the cultures were measured with the coulter counter. The cell density was determined at 0, 24, 48 and 72 hours. The growth of the control cultures fulfilled the validity criteria from OECD 201 (2006). The 72 -hour ErC10 and ErC50 are 4.7 and > 93 mg/L based on the mean measured concentrations.

This information is considered to be relevant and reliable for the further risk assessment.