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Ecotoxicological information

Short-term toxicity to fish

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Administrative data

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Description of key information

No toxicity up to the limit of water solubility (WS 1-2 mg/L)

Key value for chemical safety assessment

Additional information

The acute toxicity of Glycerides, mixed C8-10 and succinyl (CAS No. 91744-56-8) to fish was investigated in a study by Hafner (2012). This test was conducted according to OECD 203, under GLP conditions. Danio rerio was exposed for 96 hours in a limit test to a nominal loading rate of 70 mg/L (Water Accomodated Fraction (WAF)). This concentration was chosen based on the results obtained in the tests conducted on algae and aquatic invertebrates, which resulted in EL50 values of 67.6 mg/L and > 100 mg/L, respectively (loading rates). This approach is in accordance with the OECD Guidance document No. 126 “Threshold approach for acute fish toxicity” (2010). The Water Accommodated Fraction (WAF) was prepared by adding the test material into water, stirring for a period of 48 hours, followed by a sedimentation period of about 70 minutes. After the sedimentation period, the WAF was clear. Small crumbs of test item were however, observed on the surface and at the bottom of the beakers. The test solution was prepared without a further filtration step. Analytical measurements of the test concentration were performed via TOC and DOC analysis at the start and at the end of the test. The initial concentration was determined to be < 1.02 mg/L (LOQ), whereas 1.3 mg/L were reported at the end of the test. The latter value corresponds to a measured test concentration of 2.0 mg/L test material.


After 96 hours of exposure to the test substance, no mortality was reported in control or treatment group and therefore, the LL50 (96 h) was determined to be > 70 mg/L (nominal, loading rate) and > 2.0 mg/L (measured final test material concentration).


Based on the above information, no acute toxicity of Glycerides, mixed C8-10 and succinyl to fish species is expected within the limit of the water solubility (WS 1-2 mg/L).