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EC number: 244-599-9 | CAS number: 21829-50-5
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
Description of key information
The 30-min EC10 value for monoethanolamine in domestic activated sludge is >1,000 mg/L, the 16-h toxicity threshold (equivalent to an EC3 value) for citric acid is >10,000 mg/L. After correction for molecular weight the effect concentrations for tris[(2-hydroxyethyl)ammonium] citrate are >6,146 and >19,537 mg/L, respectively. The lowest value of >6,146 mg/L will be used in the assessment.
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 6 146 mg/L
Additional information
No respiration inhibition tests with activated sludge are available for tris[(2-hydroxyethyl)ammonium] citrate. Tris[(2-hydroxyethyl)ammonium] citrate is the acidic salt of monoethanolamine and citric acid. Monoethanolamine and citric acid are the starting materials for the synthesis of tris[(2-hydroxyethyl)ammonium] citrate, and the only difference is that in tris[(2-hydroxyethyl)ammonium] citrate the substances are present in their ionic forms as a monoethanolamine cation and a citrate anion. It is expected that the ecotoxicological properties of tris[(2-hydroxyethyl)ammonium] citrate shall be governed by the properties of monoethanolamine and citric acid (for more details see reporting format for the analogue approach in Appendix A.2).
A non-GLP OECD guideline 209 study with domestic activated sludge is available for monoethanolamine (BASF, 1991). The 30-min EC10 value is >1,000 mg/L, a safety factor need not to be applied to this value. Instead, the effect concentration will be corrected for molecular weight. The effect concentration for tris[(2-hydroxyethyl)ammonium] citrate is >6,146 mg/L.
For citric acid a non-GLP growth inhibition study (Bringmann and Kühn, 1977) with Pseudomonas putida. The 16-h toxicity threshold (equivalent to an EC3 value) is >10,000 mg/L, a safety factor need not to be applied to this value. Instead, the effect concentration will be corrected for molecular weight. The effect concentration for tris[(2-hydroxyethyl)ammonium] citrate is >19,537 mg/L.
The effect concentration of >6,146 mg/L will be used in the assessment.
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