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EC number: 608-681-1 | CAS number: 319-24-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP- and Guideline-Study with no or minor deficiencies.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 004
- Report date:
- 2004
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Version / remarks:
- EC Directive 92/69/EC Method C.3. Official Journal of the European Communities No. L383A/179
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- methyl 2-amino-5-fluorobenzoate
- EC Number:
- 608-681-1
- Cas Number:
- 319-24-4
- Molecular formula:
- C8 H8 F N O2
- IUPAC Name:
- methyl 2-amino-5-fluorobenzoate
- Test material form:
- other: yellowish solid
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- Stock solution: 200 mg/L, freshly prepared with dilution water
Dispersion treatment: 2 min ultrasound at room temperature
Solvent: None
Test concentrations: 3.13 - 6.25 -12.5 - 25 - 50 -100 mg/L
Light intensity: Nominal:60 -120 µE/M2*S Actual: mean value: 62.4 µE/m2*s
Test design: Three replicates per test concentration and six control replicates
Test organisms
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- Desmodesmus subspicatus CHODAT SAG 86.81; fresh stocks were prepared every month on Z-Agar; Light intensity amounted 35 - 70 ME/m2*s for 24 h per day
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Post exposure observation period:
- After 72 hours 0.5 ml algae suspension from the concentrations 25-100 mg/L and control were transferred to 10 ml untreated dilution water and allowed to grow further 4-7 days to determine wether the effect of the test item was reversible.
Test conditions
- Test temperature:
- 23 ± 2 °C
- pH:
- 8.02-8.59
- Nominal and measured concentrations:
- Nominal concentrations:
3.13- 6.25-12.5,25-50-100 mg/L - Details on test conditions:
- 24 hours/d light
- Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate p.a. (MERCK).
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- other: ErC50
- Effect conc.:
- 21.6 - 24.7 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- other: ErC50
- Effect conc.:
- 30.8 - 34.1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 25 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: biomass and growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 12.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: biomass and growth rate
- Results with reference substance (positive control):
- The acute toxicity of potassium dichromate to the unicellular freshwater green alga Desmodesmus subspicatus was determined over a period of 72 h from November 25 to 28, 2003. The EC50 -values of the reference item potassium dichromate after 72 h met the validity criteria according to EC Directive 92/69/EC Method C.3 Annex 2 (prescribed ranges are 0.20 -0.75 mg/L and 0.60 -1.03 mg/L potassium dichromate for inhibition of biomass growth and rate-related inhibition, respectively).
- Reported statistics and error estimates:
- NOEC and LOEC were determined by calculation of statistical significance of biomass integrals and growth rates. The BONFERRONI t-test was carried out for the determination of statistically significant differences compared to control replicates. When running a BONFERRONI t-test a Normality test and Homogeneity test were done first. P-values for both were 0.05. The a-value for BONFERRONI t-test was a=0.05.
Ebc50-values. ErC50-values and confidence intervals after 72 h were calculated by probit analysis. Probit values were taken from WEBER (1986). Confidence intervals were calculated according to a standard procedure (BREITIG &TUMPLING 1982).
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- In this study 2-Amino-5-fluorbenzoesauremethylester was found to inhibit the growth of the freshwater green alga Desmodesmus subspicatusat nominal concentrations > 25 mg/L (biomass growth and growth rate). The EC50-values with 95 % confidence intervals for inhibition of biomass growth (EbC50) and specific growth rate (ErC50) after 72 h were 23.1 (21.6 - 24.7) and 32.4 (30.8 - 34.1) mg/L, respectively.
Inhibition effects were found to be reversible at 25 to 100 mg/L. All effect levels are given based on nominal concentrations of the test item 2-Amino-5-fluorbenzoesauremethylester.
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