2,5-bis(p-toluidino)terephthalic acid

Toxicity to microorganisms

Administrative data

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: The test was conducted according to relevant guidelines. The results are plausible, however, insufficiently documented.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1994

Report date:

1994

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)

Test guideline 2

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

2,5-bis(p-toluidino)terephthalic acid

EC Number:

233-651-6

EC Name:

2,5-bis(p-toluidino)terephthalic acid

Cas Number:

10291-28-8

Molecular formula:

C22H20N2O4

IUPAC Name:

2,5-bis[(4-methylphenyl)amino]benzene-1,4-dicarboxylic acid

Details on test material:

Name: Toluidinosäure TR 100
Chemical name: 1,4 —Benzenedicarboxylic acid, 2,5 —bis[(4 — methylphenyl)aminol-2,5 —bis(p —toluidino)terephthalic acid

CAS: 010291 —28 —8
Chemical formula: C22 H20 O4 N2

Sampling and analysis

Analytical monitoring:

not required

Details on sampling:

The submission substance was tested excluding exposure to light by wrapping the test vessels with aluminium foil.

Test solutions

Details on test solutions:

pH values before start of incubation were between 7.2 and 7.3.
Because of the poor solubility of the test item, no stock solution had been prepared. Instead, the test item was added directly into the test vessels.
The test concentrations were between 500 mg/L and 1000 mg/L (no further data).

Test organisms

Test organisms (species):

activated sludge, domestic

Details on inoculum:

Amount: 1600 mg/L (dry weight)
Source: municipal sewage treatment works of Frankfurt/M-Sindlingen
Pretreatment: according to the guideline
Acclimatization: Not preadapted in the laboratory to the sumission substance or the reference substance

Test conditions

Test temperature:

20°C +- 2°C

pH:

7.2 - 7.3 before start of the test

Nominal and measured concentrations:

Nominal concentrations between 500 and 1000 mg/L

Details on test conditions:

In conducting the test, the EEC-Directive had been followed.

Reference substance (positive control):

yes

Remarks:

3,5-dichlorophenol: 5, 20, 30 mg/L

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

3 h

Dose descriptor:

other: EC20

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Effect concentrations 2

Duration:

3 h

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Effect concentrations 3

Duration:

3 h

Dose descriptor:

other: EC80

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Details on results:

The submission substance is not harmful to activated sludge micororganisms. The EC50 is in the range of >1000 mg/L (nominal concentration).

Results with reference substance (positive control):

The EC50 (3 hours) for the reference substance was in the required range of the guideline.

Reported statistics and error estimates:

no data

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

In an activated sludge respiration inhibition test, the submission substance proved to be innooxious to activated sludge microorgansims. The EC50 (3 hours) for respiration inhibition had been determined to be >1000 mg/L.

Executive summary:

In an activated sludge respiration inhibition test, the submission substance proved to be nontoxic to activated sludge microorgansims. The test was conducted according to relevant guidelines. However, documentation is insufficient (reliability 2). The EC50 (3 hours) for respiration inhibition had been determined to be >1000 mg/L.