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Ecotoxicological information

Short-term toxicity to fish

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Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2002
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: No analytical measurements of test material. TOC analyticals did not show gradient

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2002
Report Date:
2002

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
not specified
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Type:
Constituent

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Water accommodated fraction (WAFs)
Test material was added to 21 L of water and stirred for 48 hours. The stir rate was set to form a slight dimple at the water surface. After stirring, the mixture was allowed to stand for 4 hours. A wide bore glass tube, covered at one end with Parafilm was submerged into the vessel, sealed end down, to a depth of approximately 5 cm from the bottom of the vessel. The aqueous phase or WAF was removed by mid-depth siphoning to give the necessary loading rates.

- Evidence of undissolved material (e.g. precipitate, surface film, etc): Microscopic inspection of the WAFs showed no micro-dispersions or undissolved test material

Test organisms

Test organisms (species):
Oncorhynchus mykiss (previous name: Salmo gairdneri)
Details on test organisms:
TEST ORGANISM
- Common name: Rainbow trout
- Source: Brow Well Fisheries Limited, Hebden, Yorkshire, UK
- Age at study initiation (mean and range, SD):
- Length at study initiation (length definition, mean, range and SD): 4.3 cm
- Weight at study initiation (mean and range, SD): 1.07 g
- Food type: Commerical trout pellets. Feeding was discontinued 24 hours prior to start of the study. Food was held for 24 hours.

ACCLIMATION
- Acclimation period: Two weeks
- Acclimation conditions (same as test or not): same as test conditions

Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Post exposure observation period:
none

Test conditions

Hardness:
100 mg/l as CaCO3
Test temperature:
14 degrees C
pH:
7.6 - 8.0
Dissolved oxygen:
9.0 - 9.8 mg Oxygen/L
Salinity:
not applicable/freshwater
Nominal and measured concentrations:
Nominal: 1.0, 1.8, 3.2, 5.6, 10.0 mg/L

Details on test conditions:
TEST SYSTEM
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: 20 litre glass vessels
- Aeration: None

- Renewal rate of test solution (frequency/flow rate): Daily
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1




TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Dechlorinated laboratory tap water passed through activated carbon filter
- Total organic carbon: 2 mg C/L
- Particulate matter: 0.10 mg/L

- Alkalinity: 104 mg/l as CaCO3

- Conductivity: 473 uS


OTHER TEST CONDITIONS
- Photoperiod: 16 hours light


TEST CONCENTRATIONS
- Justification for using less concentrations than requested by guideline:
- Range finding study
- Test concentrations: 1.0, 10, 100, and 1000 mg/L
- Results used to determine the conditions for the definitive study:
1.0 mg/L: 0/3
10, 100, and 1000 mg/L: 3/3 for each dose
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Duration:
96 h
Dose descriptor:
LL50
Effect conc.:
4.4 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: 4.4 - 4.6 mg/L
Duration:
96 h
Dose descriptor:
NOELR
Effect conc.:
3.2 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
Behavioural abnormalities: Loss of equilibrium in 5.6 mg/L Test group at 48 hours
- Mortality of control: 0/10

Applicant's summary and conclusion

Validity criteria fulfilled:
yes