Zinc bis[O-(2-ethylhexyl)] bis[O-(isobutyl)] bis(dithiophosphate)

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

disregarded due to major methodological deficiencies

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

other: This study was designed to test adverse effects of the thermal degredation products of the test material but not the material itself. No LC50 determined.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1986

Report date:

1986

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 403 (Acute Inhalation Toxicity)

GLP compliance:

yes

Test type:

standard acute method

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Phosphorodithioic acid, mixed O,O-bis(sec-Bu and 1,3-dimethylbutyl) esters, zinc salts

EC Number:

272-238-5

EC Name:

Phosphorodithioic acid, mixed O,O-bis(sec-Bu and 1,3-dimethylbutyl) esters, zinc salts

Cas Number:

68784-31-6

Molecular formula:

C(16-24) H(36-42) O4 P2 S4 Zn

IUPAC Name:

Phosphorodithioic acid, mixed O,O-bis(sec-Bu and 1,3-dimethylbutyl) esters, zinc salts

Details on test material:

Thermal decomposition of test material containing an average of:
- 102 ppm hydrogen sulfide
- 56 ppm 2-butanethiol
- 7 ppm 4-methyl-2-pentanethiol

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source:
- Age at study initiation: 80 days
- Weight at study initiation: 373-411 g (males); 250-283 (females)
- Fasting period before study:
- Housing: Individually
- Diet (e.g. ad libitum): ad libitum
- Water (e.g. ad libitum): ad libitum
- Acclimation period: 35 days


ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22-24
- Humidity (%): 33-62
- Air changes (per hr):
- Photoperiod (hrs dark / hrs light): 12 hours


IN-LIFE DATES: From: To:

Administration / exposure

Route of administration:

inhalation: vapour

Type of inhalation exposure:

whole body

Vehicle:

other: unchanged (no vehicle)

Details on inhalation exposure:

GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
- Exposure apparatus: Hazleton 27 in cubical chambers with pyramidal tops and bottoms
- Exposure chamber volume:
- Method of holding animals in test chamber:
- Source and rate of air:
- Method of conditioning air:
- System of generating particulates/aerosols:
- Method of particle size determination:
- Treatment of exhaust air:
- Temperature, humidity, pressure in air chamber:


TEST ATMOSPHERE
- Brief description of analytical method used:
- Samples taken from breathing zone: yes/no


VEHICLE
- Composition of vehicle (if applicable):
- Concentration of test material in vehicle (if applicable):
- Justification of choice of vehicle:
- Lot/batch no. (if required):
- Purity:


TEST ATMOSPHERE (if not tabulated)
- Particle size distribution:
- MMAD (Mass median aerodynamic diameter) / GSD (Geometric st. dev.):


CLASS METHOD (if applicable)
- Rationale for the selection of the starting concentration:

Analytical verification of test atmosphere concentrations:

yes

Duration of exposure:

240 min

Concentrations:

102 ppm hydrogen sulfide
56 ppm 2-butanethiol
7 ppm 4-methyl-2-pentanethiol

No. of animals per sex per dose:

8 male and 8 female

Control animals:

yes

Details on study design:

- Duration of observation period following administration: 14 days (or other?)
- Frequency of observations and weighing: 2, 7, and 11 days
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight,organ weights, gross pathology

Results and discussion

Mortality:

None

Clinical signs:

other: Squinted or closed eyes. Labored breathing in two males.

Body weight:

No change between exposed and control

Gross pathology:

No gross pathologic changes were attributed to the exposure.

Any other information on results incl. tables

No mortality was observed during the study. During the exposure, squinted or closed eyes were observed in all exposed animals and two exposed males showed slightly labored breathing near the end of the exposure. There were no signs of toxicity observed in exposed animlas following the exposure or in control animlas throughout the study. There were no significant differences in mean body weights between exposed and control animals for either sex, 2, 7, or 11 days following the exposure.

No gross pathologic changes that could be attributed to the exposure were observed at necropsy following an 11 -day expsoure period.

Applicant's summary and conclusion

Interpretation of results:

other: Study did not evaluate substance being registered but rather thermal degradation products of substance being registered. No lethal dose or benchmark dose was established.

Remarks:

Criteria used for interpretation of results: other: This study cannot be used for classification purposes

Conclusions:

The acute toxicity of the degredation products of the test material does not appear to be substantially greater than hydrogen sulfide.

Executive summary:

The intent of the study was to measure the thermal decomposition products from phosphorodithioc acid, mixed O,O-bis(sec-butyl and 1,3 -dimethylbutyl) esters, zinc salts. Degradation results in formation of hydrogen sulfide and other gaseous products that may be of toxicological significance. The concentration of hydrogen sulfide was held at or around 100 ppm, below the minimum lethal concentration, to examine whether any unexcpected toxicity or lethality would occur from other materials.