[R-(R*,R*)]-α-[1-(methylamino)ethyl]benzyl alcohol

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

migrated information: read-across based on grouping of substances (category approach)

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study with acceptable restrictions.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1989

Report date:

1989

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: DIN 38412, part L15

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Pseudoephedrine hydrochloride

EC Number:

206-462-1

EC Name:

Pseudoephedrine hydrochloride

Cas Number:

345-78-8

IUPAC Name:

2-(methylamino)-1-phenylpropan-1-ol hydrochloride

Details on test material:

- Test substance: (+)-Pseudoephedrine hydrochloride
- Physical state: solid

Sampling and analysis

Analytical monitoring:

no

Test solutions

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION:
- Method: The test substance was added to the test water without any pretreatment

Test organisms

Test organisms (species):

Leuciscus idus

Details on test organisms:

TEST ORGANISM
- Common name: Golden orfe
- Source: Fischzucht Paul Eggers, Hohenwestedt, Germany
- Age at study initiation (mean and range, SD): 3 days
- Length at study initiation (length definition, mean, range and SD): 7.0 cm (range: 6.0 - 8.5 cm)
- Weight at study initiation (mean and range, SD): 4.4 g (range: 2.8 - 7.1 g)

HOUSING:
- Flow-through tank in tap water cleaned by active carbon and aerated with oil-free air.
- Photoperiod: 16 hours light and 8 hours dark
- Duration of housing and adaptation: about 2 months
- Water temperature: 19 - 22 °C
- Mortality during the last 2 weeks of housing: about 0.07%
- Mortality during the adaptation period: 0%
- Medical treatment: twice with 0.05 mg/L malachite green chloride; once with 10 mg/L tetracycline hydrochloride
- Type and amount of food: Growing Feed F/B 50, Ssniff Spezialdiaeten GmbH, Soest, Germany; ad libitum

ACCLIMATION:
- Adaptation to test water and test temperature: 3 days

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Hardness:

2.5 mmol/L

Test temperature:

20 °C

pH:

7.3 - 7.6

Dissolved oxygen:

7.9 - 8.4 mg/L

Nominal and measured concentrations:

- Nominal test substance concentrations: 0 (control), 100, 215, 464 and 1000 mg/L

Details on test conditions:

TEST SYSTEM:
- Test vessel: all-glass aquarium (30 x 22 x 24 cm)
- Fill volume: 10L
- Aeration: Continuous (slight) aeration with oil-free air
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1

TEST MEDIUM / WATER PARAMETERS:
- Source/preparation of dilution water: reconstituted freshwater according to DIN 38 412, part 11

OTHER TEST CONDITIONS:
- Photoperiod: 16:8 h day-night regime
- Withdrawal of food: 1 day before and during exposure

EFFECT PARAMETERS MEASURED:
- Mortality and symptoms of intoxication after 1, 4, 24, 48, 72 and 96 hours

Reference substance (positive control):

yes

Remarks:

Chloroacetamide

Results and discussion

Effect concentrations

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 460 - 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- Mortality: After 96 hours exposure 2/10 fish had died at a test concentration of 464 mg/L. All fish (10/10) had died at the highest tested concentration of 1000 mg/L. The 96-h LC50 was determined at > 464 and < 1000 mg/L.

- Other symptoms: After 4 hours exposure tumbling was observed at a test substance concentrations of 1000 mg/L . At 464 mg/L tumbling was observed after 24 hours.

Results with reference substance (positive control):

48-h LC50: ca. 31 mg/L (determined October 1988)

Reported statistics and error estimates:

Finney, D.J., Probit analysis, Cambr. Univ. press, 3rd ed., 1971. Certain aspects of this method were modified.