Benzyltoluene

Endpoint summary

Additional information

Available data for aquatic toxicity:

Short-term toxicity to fish:

SAS-40

In a 96-h acute toxicity study, zebra fish (Danio rerio) were exposed to SAS-40 at nominal concentrations of 0 (control and solvent control), 1, 2.24, 5, 11.18 and 25 mg/L under static conditions. The LC50 based on the nominal concentration was 8.4 mg/L. The results have to be interpreted with caution since the LC50 is on the limit of water solubility of SAS-40.

SAS-296:

In a 96-h acute toxicity study, rainbow trout (Oncorhynchus mykiss) were exposed to SAS-296 at nominal concentrations of 0 (control and solvent control) and 0.56 mg/L under static conditions. The LD50 was greater than the solubility limit of 0.56 mg/l. Water concentrations were not measured and the poor solubility of test substance requires a careful interpretation of test results.

SAS-296:

The toxicity of 1,4-dimethyl-2-(1-phenylethyl) was determined in a freshwater fish, Orizias latipes according to OECD 203. A 96h LC50 of 0.31 mg/L was reported

Short-term toxicity to aquatic invertebrates:

1,1 -DPE:

Twenty daphnia were exposed to the test item for 48 hours in a closed static test system. Two methods of test solution preparation were used. The first experiment was performed using a saturated solution and dilutions of the saturated solution. The second experiment was performed using test solutions which were prepared through spiking with an acetonic solution of the test item. The LC50 was estimated as 1.2 mg/l for saturated solutions and 1.4 mg/l for spiked solutions respectively.

In a second study with 1,1 -DPE with Klimisch score 3 no analytical verification of test substance concentration could be performed. As the test substance is only sparingly soluble in water and no dose verification was possible, the study reliability can only be judged as 3.

Phenyltolylethane

In a 48-h acute toxicity study, water fleas (Daphia magna) were exposed under static conditions

to analytically measured test concentrations of 0 (control), 0.032, 0.078, 0.18, 0.55, 0.96, 1.9 and 3.9 mg/L PTE. The 48h-EC50 was estimated as 0.29 mg/l.

SAS-40:

In a 48-h acute toxicity study, water fleas (Daphia magna) were exposed to SAS-40 at nominal concentrations of 0 (control and solvent control), 0.01, 0.018, 0.032, 0.056, 0.10, 0.18, 0.32, 0.56 and 1 mg/L under static conditions. Analytical verification of test concentrations showed a marked decline. Therefore, results should be interpreted with caution and effect levels should be expressed based on measured concentrations. The EC50 for immobilization at 48 hours was 0.32 mg/l.

SAS-296:

In a 48-h acute toxicity study, water fleas (Daphia magna) were exposed to SAS-296 at nominal concentrations of 0 (control and solvent control), 0.0056, 0.01, 0.018, 0.032, 0.056, 0.10, 0.18, 0.32 and 0.56 mg/L under static conditions. Water stability under test conditions was not addressed in this study. The 48h EC50 was estimated as 0.39 mg/l.

SAS-296:

For daphnids, a 48 h EC50 of 0.25 mg/L was reported for Daphnia magna. The study was performed with 1,4-dimethyl-2-(1-phenylethyl) according to OECD 202.

SAS-305:

In a 48-h acute toxicity study, water fleas (Daphia magna) were exposed to SAS-305 at measured concentrations of 0 (Blank and dilution water control) and 1.27±0.39 µg/L under static conditions. The 48h EC50 was estimated to be >1.27 µg/L, which was the highest test concentration measured in a saturated solution.

Benzyltoluene:

Twenty daphnia were exposed to the test item for 48 hours in a closed static test system. Two methods of test solution preparation were used. The first experiment was performed using a saturated solution and dilutions of the saturated solution. The second experiment was performed using test solutions which were prepared through spiking with an acetonic solution of the test item. The LC50 was estimated as 0.94 mg/l for saturated solutions and 1.0 mg/l for spiked solutions respectively.

Faradol 810 (without epoxide):

Twenty daphnia were exposed to the test item for 48 hours in a static test system. Acetone was used as solvent to prepare the test solutions. The 48h EC50 was estimated as 1.2 mg/l.

Faradol 810 (with epoxide):

Twenty daphnia were exposed to the test item for 48 hours in a static test system. Acetone was used as solvent to prepare the test solutions. The 48h EC50 was estimated as 1.6 mg/l.

Toxicity to aquatic algae:

SAS-296:

Scenedesmus subspicatus was exposed to an aqueous solution of the test material at a concentration of 0.56 mg/l (six replicate flasks) for 72 hours. No analytical verification of test substance concentrations was performed. The EC50 value was estimated as >0.56 mg/l.

SAS-296:

Selenastrum capricornutum was exposed to 1,4-dimethyl-2-(1-phenylethyl) for 72 hours. The test was undertaken using a dispersant (HCO-50) at a concentration of 40 mg/L. The toxicity values EC50 based on mean measured concentrations were >1.54 mg/L and 0.93 mg/L, respectively.

Toxicity to microorganism:

Phenyltolylethane:

The influence of Phenyl-tolyl-ethane on the respiration rate of activated sludge was investigated after a contact time of 3 hours according to OECD guideline 209. The EC50 was above the highest loading rate tested (1000 mg/l).