Dihexadecyl peroxodicarbonate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Study period:

1989-10-18 to 1989-11-11

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Remarks:

No chemical analysis due to poor solubility

Justification for type of information:

See attached read across document.

Reason / purpose for cross-reference:

read-across source

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

1984

Deviations:

yes

Remarks:

The study was performed without analytics.

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Version / remarks:

1984

Deviations:

yes

Remarks:

The study was performed without analytics.

GLP compliance:

yes

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: 1000 mg of the test item to 1 L of diluting water and subsequent stirring for 24 hours at room temperature to reach an equilibrium concentration of the test substance in the test substance in the test medium
- Eluate: diluting water
- Controls: yes

Test organisms (species):

Poecilia reticulata

Details on test organisms:

TEST ORGANISM
- Common name: Poecilia reticulata (guppy)
- Source: IOB (Instituut voor Onderzoek van Bestrijdingsmiddelen), Wageningen, The Netherlands
- Age at study initiation: > 4 weeks
- Length at study initiation: approximately 2 cm

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Hardness:

13°dH

Test temperature:

22 - 26 °C

pH:

7.5 to 8.2

Dissolved oxygen:

5.9 to 7.8 mg/L

Nominal and measured concentrations:

- nominal concentration: 1000 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 2 L covered glass aquaria which contained 1 L of the test medium
- Type (delete if not applicable): closed
- No. of organisms per vessel: 10 fishs in the control vessel and in the test vessel, whereas the duplicate test vessel contained 11 fishs
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 1
- Biomass loading rate: 0.6 g biomas/L

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: a synthetic water, Dutch Standard Water according NPR 6507 (1980)
- Conductivity: less than 5 µS/cm
- Intervals of water quality measurement: Measurements of oxygen concentration were carried out on days 2 and 4, pH-measurements on days 0, 2 and 4. If a high mortality was observed on the other days, the oxygen concentrations and pH were measured then also.

OTHER TEST CONDITIONS
- Photoperiod: 12 hours per day

EFFECT PARAMETERS MEASURED: The surviving fish were counted daily and dead fish removed immediately upon observation.

TEST CONCENTRATIONS
- Range finding study: yes
- Test concentrations: 1, 10, 1000 mg/L
- Results used to determine the conditions for the definitive study: No mortality was observed.

Reference substance (positive control):

no

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

The fish survived in a saturated solution containing 1 g the test item /L for 96 hours. Binormal theory dictates that when 10 fish are used with 0 % mortality, there is a 99.9 % confidence that the LC50 (96 h) is greater than 1000 mg/L. However, given that during the test flakes of the test item remained visible at the bottom of the vessel, the LC50 should be handled with care.
No effects such as deviations in the behavior or appearance of the fish were not observed.

Validity criteria fulfilled:

yes

Conclusions:

The LC50 (96 h) of ditetradecyl peroxydicarbonate is greater than 1000 mg/L.

Executive summary:

Ditetradecyl peroxydicarbonate was tested in an acute toxicity test with Poecilia reticulata (guppy) under semi-static conditions in accordance with EU method C.1 and OECD guideline No. 203. The test was performed in duplicate. The fish survived for four days in a saturated solution which contained 1000 mg of the test substance/L. Binormal theory dicates that when 10 fish are used showing 0 % mortality, there is a 99.9 % confidence that the LC50 (96 h) is greater than 1000 mg/L. However, given that during the test flakes of the test substance remained visible at the bottom of the vessel, the LC50 value should be handled with care. No deviations in the behaviour or appearance of the fish were observed.

Description of key information

One standard OECD 203 study is available for the closely related material Dimyristiylperoxydicarbonate. In this study the LC50 was not reached and was therefore higher than the highest tested concentration of 1000 mg/L. This result is supported by an OECD 236 for Dimyristiylperoxydicarbonate as well as an OECD 236 with Dicetylperoxydicarbonate.

For additional information see the accompanying readacross document. Due to the physical chemical properties of these material being similar and the toxicity to fish embryos for both materials both being above the solubility limits of both materials. It is considered justified to read across to the existing adult fish endpoint for Dimyristiylperoxydicarbonate. This study has therefore been highlighted as the key information for this endpoint. The LC50 was not reached in any of the three available data points. Due to greater than values (>) not being acceptable in the fields below the LC50 has been entered as 1000 mg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

1 000 mg/L

Additional information

In addition, algae and daphnia endpoints (4x studies) for Dicetylperoxydicarbonate and Dimyristiylperoxydicarbonate have all showed no effects at the limit of solubility (EC50 > water solubility). In combination with the (2x) fish embryo toxicity endpoints also showing (EC50 > water solubility for fish species) and the similar physical/chemical properties and almost identical chemical structure. It is considered justified to use the existing acute adult fish test data to fulfil this endpoint and avoid further animal testing.