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EC number: 235-468-7 | CAS number: 12237-62-6 This substance is identified in the Colour Index by Colour Index Constitution Number, C.I. 42535:3.
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- Experimental test result performed according to the guideline
- Qualifier:
- according to guideline
- Guideline:
- other: EEC L 133, p. 118-122 (30. May 1988)
- Deviations:
- yes
- Remarks:
- Instead a centrifuged sludge a settled sludge was used. Due to the poor solubility of the test substance at test concentrations, no stock solution was prepared. The test substance was given directly into the medium.
- GLP compliance:
- yes
- Analytical monitoring:
- not specified
- Vehicle:
- not specified
- Test organisms (species):
- other: Activated sludge from a sewage treatment plant.
- Details on inoculum:
- Test System : Activated sludge collected from the sewage treatment plant of CH-4106 Therwil on 16/10/89. The preparation was carried out according to the method described in the guideline. The sludge was separated from the aqueous layer by settling instead of centrifugation.
- Test type:
- not specified
- Water media type:
- not specified
- Total exposure duration:
- 3 h
- Test temperature:
- 20 ± 2°C
- Nominal and measured concentrations:
- Reference substance : 32, 10 and 3.2 mg/L
Test substance : 108.7; 36.05; 14.65; 5 and 1.25 mg/L
21.74, 7.21, 2.93, 1 and 0.25 mg of the test substance were weighed and added to the test medium. The volume was adjusted to 200 ml with water and aerated for 3 hours.
The sludge concentration in the test bottles was 1.6 g/L. - Details on test conditions:
- Test system : Activated sludge from a sewage treatment plant.
Duration : 3 hours
Temperature : 20 ± 2°C
Vessels : 250 ml BOD flasks with gas inlet
Water : Dechlorinated drinking water
Nutrient solution : 16 g Peptone
11 g Meat extract
3.0 g Urea
0.7 g Nacl
0.4 g CaClz∙2 H2O
0.2 g MgSO4∙7 H2O
2.8 g K2HPO4
per liter dechlorinated drinking water - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol
- Key result
- Duration:
- 3 h
- Dose descriptor:
- IC50
- Effect conc.:
- 87 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Key result
- Duration:
- 3 h
- Dose descriptor:
- other: IC20
- Effect conc.:
- 5 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Key result
- Duration:
- 3 h
- Dose descriptor:
- other: IC80
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Details on results:
- Graphically determined for the test substance:
IC50 = 87 mg/L
IC20 = 5 mg/L
IC80 = > 100 mg/L - Results with reference substance (positive control):
- Graphically determined for the reference substance: IC50 = 14 mg/L
- Reported statistics and error estimates:
- Measurements : Oxygen consumption per hour in mg/liter with a ORION - Electrode Type 97-08, ORION Microprocessor Ionalizer 901 and plotted on a recorder.
- Validity criteria fulfilled:
- not specified
- Conclusions:
- Inhibitory concentrations for respiration of the bacteria were graphically determined based on nominal concentrations and values were found as below:
IC50 = 87 mg/L
IC20 = 5 mg/L
IC80 = > 100 mg/L - Executive summary:
Toxicity of test chemical was studied on microorganisms. The study was performed according to EEC method. Activated sludge collected from the sewage treatment plant of CH-4106 Therwil on 16/10/89 was used as a test bacteria. The preparation was carried out according to the method described in the guideline. The sludge was separated from the aqueous layer by settling instead of centrifugation. The sludge concentration in the test bottles was 1.6 g/L. 21.74, 7.21, 2.93, 1 and 0.25 mg of the test substance were weighed and added to the test medium. The volume was adjusted to 200 ml with water and aerated for 3 hours. Study was carried out in a 250 ml BOD flasks with gas inlet at a temperature of 20 ± 2°C for 3 hrs. 3,5-Dichlorophenol was used as a reference substance. The effectes were observed on the respiration of aerobic waste water bacteria for 3 hours. Graphically determined IC50 value for the reference substance was evaluated to be 14 mg/l. Inhibitory concentrations for respiration of the bacteria were graphically determined based on nominal concentrations. Thus, the IC20, IC50 and IC80 value was determined to be 5, 87 and > 100 mg/l, respectively.
Reference
Description of key information
Toxicity of test chemical was studied on microorganisms (Experimental study report, 1989). The study was performed according to EEC method. Activated sludge collected from the sewage treatment plant of CH-4106 Therwil on 16/10/89 was used as a test bacteria. The preparation was carried out according to the method described in the guideline. The sludge was separated from the aqueous layer by settling instead of centrifugation. The sludge concentration in the test bottles was 1.6 g/L. 21.74, 7.21, 2.93, 1 and 0.25 mg of the test substance were weighed and added to the test medium. The volume was adjusted to 200 ml with water and aerated for 3 hours. Study was carried out in a 250 ml BOD flasks with gas inlet at a temperature of 20 ± 2°C for 3 hrs. 3,5-Dichlorophenol was used as a reference substance. The effects were observed on the respiration of aerobic waste water bacteria for 3 hours. Graphically determined IC50 value for the reference substance was evaluated to be 14 mg/l. Inhibitory concentrations for respiration of the bacteria were graphically determined based on nominal concentrations. Thus, the IC20, IC50 and IC80 value was determined to be 5, 87 and > 100 mg/l, respectively.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 87 mg/L
Additional information
Toxicity of test chemical was studied on microorganisms (Experimental study report, 1989). The study was performed according to EEC method. Activated sludge collected from the sewage treatment plant of CH-4106 Therwil on 16/10/89 was used as a test bacteria. The preparation was carried out according to the method described in the guideline. The sludge was separated from the aqueous layer by settling instead of centrifugation. The sludge concentration in the test bottles was 1.6 g/L. 21.74, 7.21, 2.93, 1 and 0.25 mg of the test substance were weighed and added to the test medium. The volume was adjusted to 200 ml with water and aerated for 3 hours. Study was carried out in a 250 ml BOD flasks with gas inlet at a temperature of 20 ± 2°C for 3 hrs. 3,5-Dichlorophenol was used as a reference substance. The effects were observed on the respiration of aerobic waste water bacteria for 3 hours. Graphically determined IC50 value for the reference substance was evaluated to be 14 mg/l. Inhibitory concentrations for respiration of the bacteria were graphically determined based on nominal concentrations. Thus, the IC20, IC50 and IC80 value was determined to be 5, 87 and > 100 mg/l, respectively.
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