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EC number: 235-468-7 | CAS number: 12237-62-6 This substance is identified in the Colour Index by Colour Index Constitution Number, C.I. 42535:3.
- Life Cycle description
- Uses advised against
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- Appearance / physical state / colour
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- Endpoint summary
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- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
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- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
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- Specific investigations
- Exposure related observations in humans
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- Additional toxicological data

Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- May 1974
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- Data is from experimental report
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 974
- Report date:
- 1974
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: The procedure adopted was the technique described in the "Appraisal of the Safety of Chemicals in Foods, Drugs and Cosmetics" (1959) of the US Association of Food and Drug Officials (AFDO).
- Principles of method if other than guideline:
- An eye irritation study of test chemical was conducted in Russian breed rabbits.
- GLP compliance:
- yes
Test material
- Reference substance name:
- Ferrate(4-), hexakis(cyano-C)-, methylated 4-[(4-aminophenyl)(4-imino-2,5-cyclohexadien-1-ylidene)methyl]benzenamine copper(2+) salts
- EC Number:
- 235-468-7
- EC Name:
- Ferrate(4-), hexakis(cyano-C)-, methylated 4-[(4-aminophenyl)(4-imino-2,5-cyclohexadien-1-ylidene)methyl]benzenamine copper(2+) salts
- Cas Number:
- 12237-62-6
- Molecular formula:
- [C24N3H28] Cu2Fe(CN)6
- IUPAC Name:
- ferrate(4-), hexakis(cyano-C)-, methylated 4-[(4-aminophenyl)(4-imino-2,5-cyclohexadien-1-ylidene)methyl]benzenamine copper(2+) salts
- Test material form:
- solid
- Details on test material:
- IUPAC name: ferrate(4-), hexakis(cyano-C)-, methylated 4-[(4-aminophenyl)(4-imino-2,5-cyclohexadien-1-ylidene)methyl]benzenamine copper(2+) salts
Substance type: Organic
Physical state: Solid
Molecular Formula: [C24N3H28] Cu2Fe(CN)6
Molecular Weight: 697.54 g/mol
Constituent 1
Test animals / tissue source
- Species:
- rabbit
- Strain:
- other: Russian breed
- Details on test animals or tissues and environmental conditions:
- TEST ANIMALS
- Housing: The substance was tested on 6 rabbits (3 males/3 females) of.the Russian breed, which were kept separately in V2A wire cages
- Diet (e.g. ad libitum): fed on NAFAG, Gossau SG, rabbit food and water ad libitum
- Only rabbits showing normal ophthalmic findings were included in the test.
Test system
- Vehicle:
- unchanged (no vehicle)
- Controls:
- yes
- Amount / concentration applied:
- 0.1 g
- Duration of treatment / exposure:
- Amounts of 0.1 g of the test substance were introduced into the conjunctival sac of the left eye with a spatula. After application, the eyelids were held open for a few seconds. The treated eyes of three rabbits were each rinsed with 10 ml lukewarm water about 30 seconds after treatment
- Observation period (in vivo):
- The reactions were appraised with a slit-lamp after 24 hours and after 2, 3, 4 and 7 days
- Number of animals or in vitro replicates:
- 6 (3 males/3 females)
- Details on study design:
- REMOVAL OF TEST SUBSTANCE
- Washing (if done): The treated eyes of three rabbits were each rinsed with 10 ml lukewarm water about 30 seconds after treatment.
Procedure : Amounts of 0.1 g of the test substance were introduced into the conjunctival sac of the left eye with a spatula.After application, the eyelids were held open for a few seconds. The right eye served as a control. The treated eyes of three rabbits were each rinsed with 10 ml lukewarm water about 30 seconds after treatment.
TOOL USED TO ASSESS SCORE: slit lamp.
Results and discussion
In vivo
Results
- Irritation parameter:
- overall irritation score
- Basis:
- mean
- Time point:
- 7 d
- Score:
- 0
- Max. score:
- 110
- Reversibility:
- not specified
- Remarks on result:
- no indication of irritation
- Irritant / corrosive response data:
- The irritation index was found to be 0 for the cornea, 0 for the iris and 0 for the conjunctivae.
The total maximum is 80 for the cornea, 10 for the iris and 20 for the conjunctivae
Any other information on results incl. tables
Applicant's summary and conclusion
- Interpretation of results:
- other: Not irritating
- Conclusions:
- The primary-irritation index (overall irritation score), which serves as a measure of the acute irritation provoked by the substance, was estimated to be 0 for the cornea, 0 for the iris and 0 for the conjunctivae. Therefore the test chemical was considered as non-irritant to the eye of rabbits.
- Executive summary:
An eye irritation study of test chemical was conducted in six (3 males/3 females) Russian breed rabbits. The study was performed as per the procedure adopted was the technique described in the "Appraisal of the Safety of Chemicals in Foods, Drugs and Cosmetics" (1959) of the US Association of Food and Drug Officials (AFDO). Amounts of 0.1 g of the test substance were introduced into the conjunctival sac of the left eye with a spatula. After application, the eyelids were held open for a few seconds. The treated eyes of three rabbits were each rinsed with 10 ml lukewarm water about 30 seconds after treatment. The reactions were appraised with a slit-lamp after 24 hours and after 2, 3, 4 and 7 days. The total maximum scores are as follows: 80 for the cornea, 10 for the iris and 20 for the conjunctivae. The irritation index was estimated to be 0 for the cornea, 0 for the iris and 0 for the conjunctivae out of maximum score of 110. Since there were no evidence of any signs of ocular lesions, the test chemical was considered to be not irritating to the rabbits’ eye.
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