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EC number: 203-960-0 | CAS number: 112-33-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Comparable to guideline study with restrictions. (Only 4 test strains used and only 2-Aminoanthracene is used as positive control for S9 mix. No testing for cross-linking using TA102 or WP strains.)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 992
- Report date:
- 1992
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Version / remarks:
- adopted 21 July 1997
- Deviations:
- yes
- Remarks:
- only 4 tester strains used; no testing for crosslinking using TA102 or E.coli WP2 strains; 2-AA sole positive control used for S9 mix
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 2-[2-(3-aminopropoxy)ethoxy]ethanol
- EC Number:
- 203-960-0
- EC Name:
- 2-[2-(3-aminopropoxy)ethoxy]ethanol
- Cas Number:
- 112-33-4
- Molecular formula:
- C7H17NO3
- IUPAC Name:
- 2-[2-(3-aminopropoxy)ethoxy]ethan-1-ol
- Details on test material:
- - Name of test material (as cited in study report): 9-Amino-3,6-dioxanonan-1-ol
- Physical state: colorless liquid
- Analytical purity: 99.23 %
- Lot/batch No.: 1/9/3
- Test substance No.: 91/455
- Storage condition of test material: room temperature
Constituent 1
Method
- Target gene:
- his operon
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Additional strain / cell type characteristics:
- other: uvrB (all strains), rfa (all strains), R factor plasmid (TA98 + TA100)
- Metabolic activation:
- with and without
- Metabolic activation system:
- co-factor supplemented post-mitochondrial fraction (S9 mix), prepared from the livers of rats pretreated for 5 days with 500 mg/kg bw Aroclor 1254
- Test concentrations with justification for top dose:
- first (standard plate test) and second (preincubation test) experiment:
20, 100, 500, 2500, 5000 µg/plate
with and without metabolic activation - Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: water
Controls
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- Remarks:
- water
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: 2-Aminoanthracene (2-AA, 2.5µg/plate; all +S9); N-methyl-N'-nitro-N-N-nitroso-guanidine (MNNG, 5µg/plate, TA100 & TA 1535, -S9), 4-nitro-o-phenylendiamine chloride monohydrate (NPD, 10µg/plate; TA98 -S9); 9-Aminoacridine (AAC, 100µg/plate; TA1537 -S9)
- Remarks:
- all positive controls dissolved in DMSO
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in medium; with and without preincubation
DURATION
- Preincubation period: 20 minutes
- Exposure duration: 48 hours
NUMBER OF REPLICATIONS: 3 replications in 1 experiment each for standard plate test and preincubation test
DETERMINATION OF CYTOTOXICITY
- Method: reduced background growth and decrease in number of his+ revertants - Evaluation criteria:
- In general, a substance to be characterized as positive in the AMES Test has to fulfill the following requirements:
- doubling of the spontaneous mutation rate (control)
- dose-response relationship
- reproducibility of the results - Statistics:
- mean values and standard deviations were calculated
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- in all tester strains depending on strain and test condtions at >2500 µg/plate
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- only with TA1537 and TA98 at 5000µg/plate
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Additional information on results:
- TEST-SPECIFIC CONFOUNDING FACTORS
- Precipitation: none observed - Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'. Remarks: preincubation test
Any other information on results incl. tables
table1: Test results of experiment I (standard plate test)
With or without S9 mix |
Test substance concentration [µg/plate] |
Mean number of revertant colonies per plate (average of 3 plates ± standard deviation) |
|||
Basepair substitution type |
Frameshift type |
||||
TA100 |
TA1535 |
TA98 |
TA1537 |
||
- |
0 |
84 ± 1 |
12 ± 3 |
21 ± 3 |
8 ± 1 |
- |
20 |
103 ± 10 |
12 ± 3 |
21 ± 3 |
7 ± 1 |
- |
100 |
95 ± 5 |
13 ± 3 |
14 ± 2 |
9 ± 2 |
- |
500 |
68 ± 10 |
12 ± 4 |
15 ± 3 |
4 ± 1 |
- |
2500 |
95 ± 11 |
11 ± 2 |
18 ± 5 |
6 ± 2 |
- |
5000 |
74 ± 5 |
13 ± 2 |
14 ± 3 |
5 ± 4 |
Positive controls, -S9 |
Name
Conc. [µg/plate] Mean no. of col./plate (average of 3 ± SD) |
MNNG*
5
880 ± 36 |
MNNG*
5
740 ± 31 |
NPD*
10
777 ± 50 |
AAC*
100
602 ± 58 |
|
|||||
|
|
TA100 |
TA1535 |
TA98 |
TA1537 |
+ |
0 |
91 ± 9 |
13 ± 2 |
24 ± 4 |
9 ± 3 |
+ |
20 |
92 ± 21 |
11 ± 3 |
24 ± 3 |
7 ± 2 |
+ |
100 |
108 ± 10 |
14 ±3 |
23 ± 8 |
7 ± 2 |
+ |
500 |
109 ± 8 |
10 ± 2 |
37 ± 8 |
5 ± 1 |
+ |
2500 |
113 ± 17 |
13 ± 4 |
25 ± 4 |
4 ± 0 |
+ |
5000 |
89 ± 8 |
12 ± 2 |
11 ± 2 |
5 ± 1 |
Positive controls, +S9 |
Name
Conc. [µg/plate] Mean no. of col./plate (average of 3 ± SD) |
2-AA*
2.5
1013 ± 21 |
2-AA*
2.5
93 ± 15 |
2-AA*
2.5
835 ± 42 |
2-AA*
2.5
82 ± 7 |
table2: Test results of experiment II (preincubation test)
With or without S9 mix |
Test substance concentration [µg/plate] |
Mean number of revertant colonies per plate (average of 3 plates ± standard deviation) |
|||
Basepair substitution type |
Frameshift type |
||||
TA100 |
TA1535 |
TA98 |
TA1537 |
||
- |
0 |
100 ± 14 |
16 ± 4 |
28 ± 5 |
8 ± 1 |
- |
20 |
115 ± 11 |
15 ± 4 |
18 ± 4 |
7 ± 0 |
- |
100 |
122 ± 10 |
12 ± 3 |
23 ± 8 |
6 ± 2 |
- |
500 |
126 ± 2 |
17 ± 3 |
21 ± 7 |
8 ± 2 |
- |
2500 |
87 ± 15 |
8 ± 1 |
19 ± 1 |
3 ± 1 |
- |
5000 |
58 ± 17 |
5 ± 4 |
- ± - |
- ± - |
Positive controls, -S9 |
Name
Conc. [µg/plate] Mean no. of col./plate (average of 3 ± SD) |
MNNG*
5
578 ± 66 |
MNNG*
5
571 ± 30 |
NPD*
10
744 ± 38 |
AAC*
100
412 ± 14 |
|
|||||
|
|
TA100 |
TA1535 |
TA98 |
TA1537 |
+ |
0 |
107 ± 5 |
13 ± 3 |
27 ± 7 |
12 ± 2 |
+ |
20 |
118 ± 10 |
14 ± 2 |
24 ± 4 |
7 ± 1 |
+ |
100 |
125 ± 16 |
13 ± 1 |
27 ± 8 |
9 ± 3 |
+ |
500 |
110 ± 11 |
11 ± 2 |
27 ± 3 |
10 ± 0 |
+ |
2500 |
105 ± 20 |
12 ± 3 |
24 ± 6 |
6 ± 2 |
+ |
5000 |
104 ± 4 |
13 ± 3 |
18 ± 3 |
5 ± 2 |
Positive controls, +S9 |
Name
Conc. [µg/plate] Mean no. of col./plate (average of 3 ± SD) |
2-AA*
2.5
561 ± 9 |
2-AA*
2.5
82 ± 3 |
2-AA*
2.5
556 ± 39 |
2-AA*
2.5
78 ± 9 |
* MNNG - N-methyl-N'-nitro-N-nitroso-guanidine; NPD - 4 -nitro-o-phenylendiamine; AAC - 9 -Aminoacridine chloride monohydrate; 2AA - 2 -Aminoanthracene
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
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