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EC number: 429-530-4 | CAS number: 96662-24-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
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- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
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- Nanomaterial pour density
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- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Bioaccumulation: aquatic / sediment
Administrative data
Link to relevant study record(s)
- Endpoint:
- bioaccumulation in aquatic species: fish
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 28 October 1997 to 06 February 1998
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 305 C (Bioaccumulation: Test for the Degree of Bioconcentration in Fish)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: Chemical Substances Control Law (Japanese law 117, 1974)
- Deviations:
- no
- GLP compliance:
- yes
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
Not applicable. - Radiolabelling:
- no
- Details on sampling:
- Test water and fish were analysed on the following dates:
Day 0 Analysis of water
Day 7 Analysis of water and fish
Day 14 Analysis of water and fish
Day 21 Analysis of water and fish
Day 28 Analysis of water and fish
Day 35 Analysis of water
Day 42 Analysis of water and fish
Day 49 Analysis of water
Day 56 Analysis of water and fish
Analysis of control fish: Fish were collected and analysed at the beginning and the end of the exposure period (Days 0 and 56). - Vehicle:
- yes
- Details on preparation of test solutions, spiked fish food or sediment:
- Dispersion method
Dispersing agent: FDPS (Naphthalene sulfonic acid - formaldehyde condensation product)
Procedure: The test substance (T-9601), 2.52463 g, and FDPS which is three times as much as the test substance were ground in a mortar with a pestle (An automatic apparatus was used (SOP/TNS/4.30)). Pure water was added drop wise into the mortar when the substances began to be coagulated. After the test substance was dispersed uniformly, the water dispersion was diluted with additional water to 2.5 L (Concentration of the test substance: 1000 ppm).
Temperature: 23.7±0.1°C
Test water: De-chlorinated water (prepared from the Tokyo metropolitan tap water) - Test organisms (species):
- Cyprinus carpio
- Details on test organisms:
- Test Fish Carp (Cyprinus carpio L.)
Source; Saku-yoshoku Co., 3-5-14 Setagaya, Setagaya-ku, Tokyo, Japan
Date of purchase: September 17, 1997 (Lot No. 70917)
Acclimation: Carp of 20-40 g and about 10 cm were sorted out when received in Institute of Ecotoxicology, Gakushuin University (SOP 2.5), and they were acclimated in Aquarium No. 1 (SOP 2.5). On November 25, 1997, they were sorted out again and transferred to Aquarium No. 7. They were on medication (New Green F®, Shin Fuso Pharmaceutical Co., Ltd.) in water before the testing (SOP 2.3). Water temperature during the acclimation was gradually raised to 25°C by the start of the test (SOP 2.5).
Carp: Average body weight: 25.9 ± 4.5 g (SOP 2.8)
Average body length: 9.9 ± 0.6 cm (SOP 2.8)
Average fat content: 4.1 ± 0.5% (SOP 2.9 (2))
(measured by CHCl3-MeOH Extraction Method) - Route of exposure:
- aqueous
- Test type:
- flow-through
- Water / sediment media type:
- natural water: freshwater
- Total exposure / uptake duration:
- 8 wk
- Hardness:
- No data
- Test temperature:
- 24.8 – 25.2°C
- pH:
- 7.1 - 7.6
- Dissolved oxygen:
- 7.0 - 7.6 ppm
- TOC:
- No data
- Salinity:
- Not applicable.
- Details on test conditions:
- Aquarium:
Aquarium for flow-through test. Made of glass, Test volume of 100l Flow rate: 300ml/min. (432L/day) Aquarium No, 8 was used for high exposure level, No. 9 for low exposure level, and No. 7 for control.
Water: De-chlorinated water (prepared from the Tokyo metropolitan tap water).
Number of fish: High and low exposure levels: 25 fish each; Control: 6 fish
Temperature 25.1±0.3°C
Concentrations: The test substance in water was set at concentrations as low as possible with consideration given to both the analytical sensitivity and the 43-hr LC5O value.
Concentration of the test substance Concentration of FDPS
High exposure level 0.1 ppm (w/v) 0.3 ppm (w/v)
Low exposure level 0.01 ppm (w/v) 0.03 ppm (w/v)
Control ---- 0.3 ppm (w/v)
Analysis of Test Fish
Pre-treatment
Three fish were collected with a dip net from high or low exposure level or control on their respective dates of analysis, and led to apparent death by hitting the back of the head, the surface of which was then wiped softly with a piece of gauze, and weighed in tenths of a gram with a top-loading balance. The body length, which is defined as a distance from the tip of mouth to the base of caudal fin, was measured in tenths of a centimetre. Two of three fish each were used for analysis, and the other was wrapped with a piece of aluminium foil, and then packed in a plastic bag and placed in a plastic case before stored in a freezer.
The individual test fish was cut into pieces and placed in a homogenizer cup, to which 150 ml of dichloromethane / acetone (2/1 (v/v)) was added, and homogenized at 10000 rpm for 10 minutes. The homogenate was transferred into a 250 ml centrifuge tube. The inside of the cup was washed with six 30 ml of dichloromethane / acetone (2/1 (v/v)) and the six washings were transferred into the centrifuge tube, which was then centrifuged at 8000 rpm for 15 minutes at 15 °C. The supernatant was filtered with Advantec No. 2 filter paper, of which the filtrate was collected in a 500 ml rounded-bottom flask. After that, the filtrate was evaporated to dryness under a reduced pressure with a rotary evaporator (water bath. 25°C). The residue was dissolved with divisions of 80 ml of n-hexane and 140 ml of acetonitrile successively, and its solutions were transferred into a 300 ml separatory funnel. The funnel was shaken for 15 minutes with a shaker and left stand for a while. The acetonitrile layer (lower layer) was transferred to a 500 ml rounded-bottom flask and evaporated to dryness under a reduced pressure with the rotary evaporator (water bath: 25°C).
This extract from fish was dissolved with 2 ml of n-hexane/ acetone (9/1) and the solution was injected into a Sep-Pack Silica® cartridge column (conditioned with 5 ml of n-hexane / acetone (9/1) in advance). The inside of the flask was washed with two 1 ml of the solvent mixture, and the two washings were also injected into the column (the test substance is adsorbed to the Sep-Pack Silica). Seven millilitres of n-hexane/ acetone (8/2) was gradually added to the column, and the eluate was pooled in a 50 ml rounded-bottom flask (the test substance is eluted in this fraction). After that, the eluate was evaporated to dryness under a reduced pressure with the rotary evaporator (water bath: 25°C).
The residue was dissolved with 2 ml of acetonitrile, and the solution was transferred into a 10 ml volumetric flask. Three millilitres of pure water was also added into the flask. The inside of the rounded-bottom flask was washed with several portions of acetonitrile, and each washings was transferred into the volumetric flask, which was then filled with acetonitrile (an acetonitrile solution containing 30% water). An aliquot of this final solution was filtered with LC disk 13 CR, and the filtrate was analysed by high performance liquid chromatography (HPLC).
Analysis of Test Water
Pre-treatment
About 500 ml (about 2000 ml) of test water of high exposure level was withdrawn from the middle of the aquarium with a conical flask. The supernatant water, 200 mL (800 ml) was measured with a graduated cylinder.
The supernatant measured was transferred into a 300 ml (1l) separatory funnel. The inside of the graduated cylinder was washed with a small amount of pure water and the washings was transferred into the funnel. Dichloromethane, 80 ml (120 ml), was added into the funnel, which was then shaken for 15 minutes with the shaker and left stand for a while. The lower layer was transferred into a 300 ml rounded-bottom flask, and concentrated under a reduced pressure with the rotary evaporator. This concentration was stopped immediately before dryness, and the residual solvent was removed by forced ventilation. The residue was dissolved in a small amount of acetonitrile, and the solution was transferred into a 10 ml (5 ml) volumetric flask, into which 3 ml (1.5 ml) of pure water was then added. The inside of the rounded-bottom flask was washed with several portions of acetonitrile, and each washings was transferred into the volumetric flask, which was then filled with acetonitriie (an acetonitrile solution containing 30% water). The final solution was filtered with LC disk 13 CR, and the filtrate was analyzed by HPLC. - Nominal and measured concentrations:
- Concentration of the test substance Concentration of FDPS
High exposure level 0.1 ppm (w/v) 0.3 ppm (w/v)
Low exposure level 0.01 ppm (w/v) 0.03 ppm (w/v)
Control ---- 0.3 ppm (w/v) - Reference substance (positive control):
- not required
- Details on estimation of bioconcentration:
- The 48-hr LC50 of the test substance to orange killifish was more than 186 ppm. Accordingly, the nominal concentrations to be used in the bioconcentration test were set at 0.1 ppm and 0.01 ppm. The exposure period of the test was set at 8 weeks. We obtained the bioconcentration factors (BCFs) ranging from 15 to 58 for the 0.1 ppm exposure level and from 62 to 124 for the 0.01 ppm exposure level.
- Type:
- BCF
- Value:
- > 15 - < 58 dimensionless
- Basis:
- whole body w.w.
- Time of plateau:
- 56 d
- Calculation basis:
- steady state
- Remarks on result:
- other: Conc.in environment / dose:0.1 ppm (w/v)
- Type:
- BCF
- Value:
- > 62 - < 124 dimensionless
- Basis:
- whole body w.w.
- Time of plateau:
- 56 d
- Calculation basis:
- steady state
- Remarks on result:
- other: Conc.in environment / dose:0.01 ppm (w/v)
- Details on kinetic parameters:
- Not calculated.
- Metabolites:
- Not assessed.
- Results with reference substance (positive control):
- Not applicable.
- Details on results:
- Further information in table form submitted under any other information
- Reported statistics:
- No data
- Validity criteria fulfilled:
- yes
- Conclusions:
- From the BCFs obtained, it can be concluded that the test substance, T-9601, is not bioaccumulative in carp.
- Executive summary:
A bioconcentration study of the test substance, T-9601, with carp (Cyprinus carpio) was performed according to the standard method described in the governmental order, which prescribes the procedure of testing new chemical substances as required by the Chemical Substances Control Law (Japanese law 117, 1974). This method is also known as the "Bioaccumulation, Test for the Degree of Bioconcentration in Fish" in the OECD Guidelines for the Testing of Chemicals No.305C(1981). Study conducted in compliance with GLP.
The 48-hr LC50 of the test substance to orange killifish was more than 186 ppm. Accordingly, the nominal concentrations to be used in the bio-concentration test were set at 0.1 ppm and 0.01 ppm. The exposure period of the test was set at 8 weeks. We obtained the bio-concentration factors (BCFs) ranging from 15 to 58 for the 0.1 ppm exposure level and from 62 to 124 for the 0.01 ppm exposure level.
From the BCFs obtained, it can be concluded that the test substance, T-9601, is not bioaccumulative in carp.
On the basis of this data, the substance cannot be considered as bioaccumulative under environmentally relevant conditions. The substance is not considered to pose a chronic hazard to the aquatic environment on the basis of this data.
Reference
Bioconcentration factors
Day |
High exposure level |
Low exposure level |
||
A |
BCFs |
A |
BCFs |
|
B |
B |
|||
7 |
A |
25 |
A |
114 |
B |
15 |
B |
82 |
|
14 |
A |
43 |
A |
77 |
B |
29 |
B |
88 |
|
21 |
A |
42 |
A |
62 |
B |
37 |
B |
105 |
|
28 |
A |
31 |
A |
11 |
B |
33 |
B |
104 |
|
42 |
A |
40 |
A |
98 |
B |
44 |
B |
96 |
|
56 |
A |
51 |
A |
121 |
B |
58 |
B |
124 |
(Note 1) Fish were given the letter A or B for identification when they were collected for analysis.
Analysis of Fish (High exposure level)
Sample fish |
A Final volume
(ml) |
B Peak area |
C Concentration in final solution *
(ppm) |
D Mass detected in fish
(μg) |
E Fish weight
(g) |
F Recovery
(%) |
G Concentration in fish
(ppm) |
H Concentration in water (cumulative average) (ppm) |
I Bio-concentration factor |
|
7 |
A |
10 |
482191 |
4.7668 |
47.668 |
21.8 |
89.8 |
2.43 |
0.0965 |
25 |
7 |
B |
10 |
282028 |
2.7921 |
27.921 |
21.0 |
89.8 |
1.48 |
0.0965 |
15 |
14 |
A |
10# |
384425 |
3.8375 |
76.750 |
20.0 |
89.8 |
4.27 |
0.0983 |
43 |
14 |
B |
10# |
339163 |
3.3871 |
67.742 |
26.4 |
89.8 |
2.86 |
0.0983 |
29 |
21 |
A |
10# |
530066 |
5.1702 |
103.404 |
27.4 |
89.8 |
4.20 |
0.0992 |
42 |
21 |
B |
10# |
404617 |
3.9523 |
79.046 |
24.1 |
89.8 |
3.65 |
0.0992 |
37 |
28 |
A |
10# |
364435 |
3.6158 |
72.316 |
26.1 |
89.8 |
3.09 |
0.0998 |
31 |
28 |
B |
10# |
385580 |
3.8250 |
76.500 |
25.7 |
89.8 |
3.31 |
0.0998 |
33 |
42 |
A |
10# |
501163 |
4.9150 |
98.300 |
27.4 |
89.8 |
4.00 |
0.101 |
40 |
42 |
B |
10# |
491192 |
4.8172 |
96.344 |
23.9 |
89.8 |
4.49 |
0.101 |
44 |
56 |
A |
10# |
725263 |
7.1585 |
143.170 |
31.2 |
89.8 |
5.11 |
0.101 |
51 |
56 |
B |
10# |
746819 |
7.3711 |
147.422 |
28.2 |
89.8 |
5.82 |
0.101 |
58 |
Calculation equations: Mass detected in fish: D=CXA (X2 (Dilution factor))#
Concentration in fish: G=(DX100)/(EXF)
Bioconcentration factor: I=G/H
*Based on two-point calibration
#On day 14 and after, we diluted the final solutions twofold to determine the test substance concentration within the assay range.
Analysis of Fish (Low exposure level)
Sample fish |
A Final volume
(ml) |
B Peak area |
C Concentration in final solution*
(ppm) |
D Mass detection in fish
(μg) |
E Fish weight
(g) |
F Recovery
(%) |
G Concentration in fish
(ppm) |
H Concentration in water (cumulative average) (ppm) |
I Bio-concentration factor |
|
7 |
A |
10 |
238201 |
2.3597 |
23.597 |
24.2 |
89.8 |
1.09 |
0.00952 |
114 |
7 |
B |
10 |
146567 |
1.4557 |
14.557 |
20.7 |
89.8 |
0.783 |
0.00952 |
82 |
14 |
A |
10 |
160313 |
1.6075 |
16.075 |
24.1 |
89.8 |
0.743 |
0.00959 |
77 |
14 |
B |
10 |
161857 |
1.6228 |
16.228 |
21.3 |
89.8 |
0.848 |
0.00959 |
88 |
21 |
A |
10 |
117668 |
1.1665 |
11.665 |
21.6 |
89.8 |
0.601 |
0.00966 |
62 |
21 |
B |
10 |
234780 |
2.3035 |
23.035 |
25.4 |
89.8 |
1.01 |
0.00966 |
105 |
28 |
A |
10 |
241177 |
2.3964 |
23.694 |
24.7 |
89.8 |
1.08 |
0.00974 |
111 |
28 |
B |
10 |
204985 |
2.0383 |
20.383 |
22.5 |
89.8 |
1.01 |
0.00974 |
104 |
42 |
A |
10 |
227145 |
2.2270 |
22.270 |
26.0 |
89.8 |
0.954 |
0.00971 |
98 |
42 |
B |
10 |
224150 |
2.1976 |
21.976 |
26.2 |
89.8 |
0.934 |
0.00971 |
96 |
56 |
A |
10 |
347790 |
3.4360 |
34.360 |
32.3 |
89.8 |
1.18 |
0.00976 |
121 |
56 |
B |
10 |
330797 |
3.2685 |
32.685 |
30.0 |
89.8 |
1.21 |
0.00976 |
124 |
Calculation equations: Mass detected in fish: D=CXA
Concentration in fish: G=(DX100) / (EXF)
Bioconcentration factor: I=G/H
*Based on two-point calibration
Analysis of Fish (Control)
Sample fish |
A Final volume
(ml) |
B Peak area |
C Concentration in final solution*
(ppm) |
D Mass detected in fish
(μg) |
E Fish weight
(g) |
F Recovery
(%) |
G Concentration in fish
(ppm) |
H Concentration in water (cumulative average) (ppm) |
I Bio-concentration factor |
0 – a |
10 |
N.D. |
--- |
--- |
21.6 |
--- |
--- |
0 |
--- |
0 – b |
10 |
N.D. |
--- |
--- |
24.9 |
--- |
--- |
0 |
--- |
56 – a |
10 |
N.D. |
--- |
--- |
38.1 |
--- |
--- |
0 |
--- |
56 – b |
10 |
N.D. |
--- |
--- |
37.7 |
--- |
--- |
0 |
--- |
(Note) Fish were given the letter “a” or “b” for identification when they were collected from the aquarium.
*Based on two-point calibration
Analysis of Water (High exposure level)
Exposure period (day) |
A Final volume
(ml) |
B Peak area |
C Concentration in final solution*
(ppm) |
D Absolute amount in final solution
(μg) |
E Volume of sample water
(g) |
F Recovery
(%) |
G Concentration in water (measured value) (ppm) |
H Concentration in water (cumulative average) (ppm) |
0 |
10 |
177926 |
1.77673 |
17.673 |
200 |
96.2 |
0.0919 |
--- |
7 |
10 |
195487 |
1.9432 |
19.432 |
200 |
26.2 |
0.101 |
0.0965 |
14 |
10 |
198990 |
1.9682 |
19.682 |
200 |
96.2 |
0.102 |
0.0983 |
21 |
10 |
199986 |
1.9657 |
19.657 |
200 |
96.2 |
0.102 |
0.0992 |
28 |
10 |
198010 |
1.9693 |
19.693 |
200 |
96.2 |
0.102 |
0.0998 |
35 |
10 |
195134 |
1.9409 |
19.409 |
200 |
96.2 |
0.101 |
0.100 |
42 |
10 |
203528 |
1.9953 |
19.953 |
200 |
96.2 |
0.104 |
0.101 |
49 |
10 |
204616 |
2.0041 |
20.041 |
200 |
96.2 |
0.104 |
0.101 |
56 |
10 |
201072 |
1.9635 |
19.635 |
200 |
96.2 |
0.102 |
0.101 |
Calculation equation: Absolute amount in final solution: D=CXA
Concentration in water: G=(DX100) /(EXF)
*Based on two-point calibration
Analysis of Water (Low exposure level)
Exposure period (day) |
A Final volume
(ml) |
B Peak area |
C Concentration in final solution*
(ppm) |
D Absolute amount in final solution
(μg) |
E Volume of sample water
(g) |
F Recovery
(%) |
G Concentration in water (measured value) (ppm) |
H Concentration in water (cumulative average) (ppm) |
0 |
5 |
141866 |
1.4102 |
7.0510 |
800 |
97.4 |
0.00905 |
--- |
7 |
5 |
156212 |
1.5552 |
7.7760 |
800 |
97.4 |
0.00998 |
0.00952 |
14 |
5 |
153464 |
1.5195 |
7.5975 |
800 |
97.4 |
0.00975 |
0.00959 |
21 |
5 |
155382 |
1.5327 |
7.6635 |
800 |
97.4 |
0.00984 |
0.00966 |
28 |
5 |
158286 |
1.5763 |
7.8815 |
800 |
87.4 |
0.0101 |
0.00974 |
35 |
5 |
153818 |
1.5321 |
7.6605 |
800 |
97.4 |
0.00983 |
0.00976 |
42 |
5 |
150251 |
1.4727 |
7.3635 |
800 |
97.4 |
0.00945 |
0.00971 |
49 |
5 |
159355 |
1.5601 |
7.8005 |
800 |
97.4 |
0.0100 |
0.00975 |
56 |
5 |
156923 |
1.5305 |
7.6525 |
800 |
97.4 |
0.00982 |
0.00976 |
Calculation equations: Absolute amount in final solution: D=CXA
Concentration in water: G=(DX100) / (EXF)
*Based on two-point calibration
Description of key information
From the BCFs obtained, it can be concluded that the test substance, T-9601, is not bioaccumulative in carp.
Key value for chemical safety assessment
- BCF (aquatic species):
- 124 dimensionless
Additional information
A bioconcentration study of the test substance, T-9601, with carp (Cyprinus carpio) was performed according to the standard method described in the governmental order, which prescribes the procedure of testing new chemical substances as required by the Chemical Substances Control Law (Japanese law 117, 1974). This method is also known as the "Bioaccumulation, Test for the Degree of Bioconcentration in Fish" in the OECD Guidelines for the Testing of Chemicals No.305C(1981). Study conducted in compliance with GLP.
The 48-hr LC50 of the test substance to orange killifish was more than 186 ppm. Accordingly, the nominal concentrations to be used in the bio-concentration test were set at 0.1 ppm and 0.01 ppm. The exposure period of the test was set at 8 weeks. We obtained the bio-concentration factors (BCFs) ranging from 15 to 58 for the 0.1 ppm exposure level and from 62 to 124 for the 0.01 ppm exposure level.
From the BCFs obtained, it can be concluded that the test substance, T-9601, is not bioaccumulative in carp.
On the basis of this data, the substance cannot be considered as bioaccumulative under environmentally relevant conditions. The substance is not considered to pose a chronic hazard to the aquatic environment on the basis of this data.
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