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Administrative data

Endpoint:
adsorption / desorption: screening
Type of information:
experimental study
Adequacy of study:
key study
Study period:
26 July 2001
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Study conducted to OECD test guidance in compliance with GLP.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2001
Report date:
2001

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))
Version / remarks:
Draft method utilised at the time of study.
Deviations:
no
GLP compliance:
yes
Type of method:
HPLC estimation method
Media:
other: Soil and Sewage Sludge using High Performance Liquid Chromatography (HPLC)

Test material

Constituent 1
Details on test material:
See below

Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
Not applicable.
Radiolabelling:
no

Study design

Test temperature:
23.5 to 24.0 °C

HPLC method

Details on study design: HPLC method:
HPLC is performed on an analytical column packed with a commercially available cyanopropyl solid phase chemically bonded on silica. Chemicals injected on such a column move along it by partitioning between the mobile solvent phase and the cyanopropyl stationary phase. As a result of partitioning between mobile and stationary phase the test substances are retarded. The dual composition of the stationary phase having polar and non-polar sites allows for interaction of polar and non-polar groups of a molecule in a similar way as is the case for organic matter in soil matrices. This enables the relationship between the retention time on the column and the adsorption coefficient on organic matter to be established.

The adsorption coefficient (Koc) of the test substance is given as its log Koc value.
Analytical Parameters
The measurements were performed with the following HPLC conditions:
Equipment;HPLC Pump SP 8810, Spectra-Physics Inc. Differential Refractometer, Knauer Inc.

Conditions:
Stationary phase Zorbax CN, 250 x 4.6 mm, 5 urn
Mobile phase methanol / water (purified) 60/40 (v/v)
Flow velocity: 1.5 ml/min
Injection volume: 20 ul
Temperature: 23.5 to 24.0 °C
Detection: Refractive index

Calibration substances
(0) Urea (inert marker): 21.9 mg/25 ml eluent
(1) Monuron 13.5 mg/25 ml solvent
(2) Triapenthenol 17.2 mg/25 ml solvent
(3) Linuron 16.0 mg/25 ml solvent
(4) Fenthion 16.8 mg/25 ml solvent
(5) Trifluralin 14.7 mg/25 ml solvent
(6) 3,5-Dinitrobenzamide 14.2 mg/25 ml solvent

The solvent was methanol / water (purified) 70/30 (v/v).

Batch equilibrium or other method

Analytical monitoring:
no
Details on sampling:
Not applicable.
Details on matrix:
Not applicable.
Details on test conditions:
Not applicable.
Computational methods:
Not applicable.

Results and discussion

Adsorption coefficient
Type:
log Koc
Value:
4.2
Temp.:
23.5 °C

Results: HPLC method

Details on results (HPLC method):
The adsorption coefficient on soil (Koc) of T-9601 was found to be 1.58x 10E+04 (log Koc=4.2).

Results: Batch equilibrium or other method

Adsorption and desorption constants:
Not measured
Recovery of test material:
Not measured
Concentration of test substance at end of adsorption equilibration period:
Not measured
Concentration of test substance at end of desorption equilibration period:
Not measured

Any other information on results incl. tables

From the retention times of the test item T-9601 the corresponding k'-values were calculated and with the function above the log Koc of T-9601 was determined by extrapolation to:

 

t

(minutes)

k'

logk'

log Koc

logKoc(mean value)

15.42

5.734

0.758

4.22

 

 

 

 

 

4.2

14.97

5.537

0.743

4.19

 

There is no greater difference than 0.1 between the two values and the corresponding mean value

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The adsorption coefficient on soil (Koc) of T-9601 was found to be 1.58x 10 E04 (log Koc=4.2).
Executive summary:

The adsorption coefficient on soil of T-9601 was determined according OECD guidelines for the testing of chemicals, Draft Guideline No. 121: Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC)", August 1999.

 

The adsorption coefficient on soil (Koc) of T-9601 was found to be 1.58x 10E04 (log Koc=4.2).

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