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EC number: 429-530-4 | CAS number: 96662-24-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 08 December 1998 to 10 December 1998
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Study conducted to EU & OECD test guidance in compliance with GLP.
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- yes
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
Not applicable. - Analytical monitoring:
- yes
- Details on sampling:
- In order to determine the substance concentration, water samples were taken at study start and study end from the concentrations relevant for EC50 determination.
- Vehicle:
- no
- Details on test solutions:
- The test substance was weighed into a beaker, mixed with water for dilution, homogenized with the Ultra-Turrax and were then stirred for approximately 24 hours with a magnetic stirrer at a temperature of approx. 50 °C to ensure that the solubility limit of the test substance in the test water was reached. Afterwards the test batches were centrifugated and than filtered off through a cellulose filter (pore size 0.2 μm) to remove undissolved parts of the test substance. Test vessels, filter and any other material coming in contact with the saturated solution were saturated with test substance before use.
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Species: Daphnia magna - Clone 5 (IRChA, France)
Origin: Stock culture of Hoechst Marion Roussel Deutschland GmbH, ProTox
Breeding conditions: 100 mL beakers (height 90 mm, diameter 50 mm), filled with 70 mL test water (according to Elendt, M4) served as culture vessels. One daphnid was used per culture vessel. The animals were used for breeding up to an age of 42 days. The reproduction rate and the state of health of the animals were monitored daily apart from weekends. The animals were fed three times a week with monocellular green algae (Scenedesmus subspicatus). - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Post exposure observation period:
- No post exposure observation period
- Hardness:
- 2.3 mmol Ca2+ + Mg2+/l
- Test temperature:
- 20.2 – 21.3°C
- pH:
- 7.9 – 8.3
- Dissolved oxygen:
- 6.3 – 9.0 mg/l
- Salinity:
- Not applicable.
- Nominal and measured concentrations:
- Nominal test concentrations: 0/22/50/100/220 mg/L
- Details on test conditions:
- The study was conducted in a static system. The test chambers were 250 mL beakers (height 110 mm, diameter 70 mm, water level about 75 mm), each filled with 200 mL test water. The room temperature was about 21 °C. The room was illuminated by fluorescent tubes for 16 hours each day.
After the test concentrations had been prepared and water parameters were recorded, 10 daphnids were used in each test chamber. The daphnids received no feed for the entire study period.
Inspection of the daphnids took place after 24 and 48 hours and involved recording the immobility. Immobile daphnids were removed from the chambers. Those animals not able to swim within 15 seconds after gentle agitation of the test container were considered to be immobile. The water parameters were measured after adding the substance and at the end of the test. - Reference substance (positive control):
- no
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- All tested concentrations were present as clear yellow to yellow-brown solutions.
The substance concentration was determined in the 50,100 and 220 mg/L groups. Due to very low water solubility of the test substance the values determined were only in a range of 0.005 - 0.097 mg/L. Moreover in the 50 mg/L were no immobility occurred a much higher test substance concentration was observed than in the 100 mg/L group were 8 of 20 animals died. Analytical determinations showed that a by product of the test substance results from the solution process, which also is responsible for the orange discolouration of the test batches. A calculation based on comparison of the peak areas showed that the concentration of the by product corresponds with the nominal concentration steps and that it remains at a constant level throughout the exposure period. This leads to the presumption that the observed effects were (might be) caused by the by product and not from the original test substance. However, the immobility values given in this report are based on nominal concentrations of the test substance. - Results with reference substance (positive control):
- Not applicable.
- Reported statistics and error estimates:
- On the basis of the immobility rate, the EC50 value was determined after 24 by means of the probit analysis (after the method according to UNDER und WEBER, confidence limit according to FIELLER).
Probit analysis could not be carried out after 48 hours because of the immobility rate. As the factor for the concentration steps was 2.2 (third root of 10), no further concentrations were tested. The EC50 was estimated graphically. - Validity criteria fulfilled:
- yes
- Conclusions:
- Under the conditions of this test and based on nominal concentrations the EC50 of T-9601 after 48 hours was near 100 mg/L.
- Executive summary:
The study was conducted to evaluate the toxicity of T-9601 to Daphnia magna following short-term exposure.
The present study was conducted in compliance with the requirements of EEC-Guideline 92/69/EWG Annex part C Methods for the Determination of Ecotoxicity C.2 Acute Toxicity for Daphnia July 31,1992and the OECD-Guideline OECD-Guideline for testing of chemicals, Section 2 - Effects on Biotic Systems Test guideline 202 Daphnia sp., 14-day Reproduction Test(including an Acute Immobilisation Test)Adopted: April 4,1984.This study was conducted in compliance with the Principles of Good Laboratory Practice(GLP).
Toxicity of T-9601 was tested in Daphnia magna {water flea) over 48 hours in a static system.
The nominal concentrations tested were 22, 50, 100, 220 mg/L and a negative control (0 mg/L).
The chamber contents were stirred for approximately 24 hours at a temperature of approx. 50 °C to ensure that the solubility limit of the test substance in the test water was reached. Afterwards the test concentrations were centrifugated and than filtered off through a cellulose filter (pore size 0.2 μm) to remove undissolved parts of the test substance. Test vessels, filter and any other material coming in contact with the saturated solution were saturated with test substance before use.
All tested concentrations were present as clear yellow to yellow-brown solutions.
The substance concentration was determined in the 50, 100 and 220 mg/L groups. Due to very low water solubility of the test substance the values determined were only in a range of 0.005 - 0.097 mg/L. Moreover in the 50 mg/L where no immobility occurred a much higher test substance concentration was observed than in the 100 mg/L group were 8 of 20 animals died. Analytical determinations showed that a by product of the test substance results from the solution process, which also is responsible for the orange discolouration of the test batches. A calculation based on comparison of the peak areas showed that the concentration of the by product corresponds with the nominal concentration steps and that it remains at a constant level throughout the exposure period. This leads to the presumption that the observed effects were (might be) caused by the by product and not from the original test substance. However, the immobility values given in this report are based on nominal concentrations of the test substance.
In the control group no immobility was observed. Immobility was observed at 100 and 220 mg/L. The 22 and 50 mg/L group showed no effect in comparison to the negative control.
Reference
IMMOBILITY
In the control group no immobility was observed. Under the given study conditions, the following immobility rates of T-9601 were recorded after 24 and 48 hours:
Concentration (mg/l) |
Immobility |
|||
24 hours |
48 hours |
|||
Absolute |
% |
Absolute |
% |
|
22 |
0/20 |
0 |
0/20 |
0 |
50 |
0/20 |
0 |
0/20 |
0 |
100 |
1/20 |
5 |
8/20 |
40 |
220 |
12/20 |
60 |
20/20 |
100 |
|
After 24 hours |
After 48 hours |
EC0 (mg/l) |
50 |
50 |
EC50 (mg/l) |
198.0 |
Approx. 115.0 |
Confidence interval |
161.7 – 265.9 |
- |
EC100 (mg/l) |
Not determined |
220.0 |
Individual values
Concentration (mg/l) |
Test-chamber No. |
Study-start (date) |
Hours after study start |
|
24 |
48 |
|||
0 |
1a |
Dec-08-98 |
0/10 |
0/10 |
0 |
1b |
Dec-08-98 |
0/10 |
0/10 |
22 |
2a |
Dec-08-98 |
0/10 |
0/10 |
22 |
2b |
Dec-08-98 |
0/10 |
0/10 |
50 |
3a |
Dec-08-98 |
0/10 |
0/10 |
50 |
3b |
Dec-08-98 |
0/10 |
0/10 |
100 |
4a |
Dec-08-98 |
0/10 |
3/10 |
100 |
4b |
Dec-08-98 |
1/10 |
5/10 |
220 |
5a |
Dec-08-98 |
5/10 |
10/10 |
220 |
5b |
Dec-08-98 |
7/10 |
10/10 |
Description of key information
Under the conditions of this test and based on nominal concentrations the EC50 of T-9601 after 48 hours was near 100 mg/L.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Dose descriptor:
- EC50
- Remarks:
- (48h)
- Effect concentration:
- >= 100 mg/L
Additional information
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