Registration Dossier

Administrative data

Endpoint:
nanomaterial aspect ratio/shape
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2019
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
test procedure in accordance with national standard methods with acceptable restrictions

Data source

Reference
Reference Type:
other: unpublished study report
Title:
Unnamed
Year:
2019
Report date:
2019

Materials and methods

Principles of method if other than guideline:
Image analysis: manual image analysis according to REACH criteria (NanoDefine ParticleSizer)
Guidelines from NanoDefine are applied:
Method selection according to Gaillard et al., Nanoscale Adv., 2019, 1, 781
TEM image evaluation according to Verleysen et al., Materials 2019, 12, 2274; doi:10.3390/ma12142274
GLP compliance:
no
Type of method:
transmission electron microscopy
Details on methods and data evaluation:
Sample Preparation
A few mg of the previously wobbled/shaken pigment powder was dispersed in distillated water in an ultrasonic bath. A droplet of the well dispersed and diluted pigment dispersion was transferred to a TEM-grid (Cu) coated with a polymer support film (Formva).

Test material

Constituent 1
Chemical structure
Reference substance name:
N,N'-(2-chloro-1,4-phenylene)bis[4-[(4-chloro-2-nitrophenyl)azo]-3-hydroxynaphthalene-2-carboxamide]
EC Number:
252-772-5
EC Name:
N,N'-(2-chloro-1,4-phenylene)bis[4-[(4-chloro-2-nitrophenyl)azo]-3-hydroxynaphthalene-2-carboxamide]
Cas Number:
35869-64-8
Molecular formula:
C40H23Cl3N8O8
IUPAC Name:
4-[(E)-2-(4-chloro-2-nitrophenyl)diazen-1-yl]-N-(3-chloro-4-{4-[(E)-2-(4-chloro-2-nitrophenyl)diazen-1-yl]-3-hydroxynaphthalene-2-amido}phenyl)-3-hydroxynaphthalene-2-carboxamide
Test material form:
solid: nanoform

Data gathering

Instruments:
Zeiss EM910 100kV
Calibration:
no data
Reproducibility:
no data

Results and discussion

Shape description
Key result
Shape:
rod
Remarks on result:
other: The material is assigned to the “elongated” shape category.
Aspect ratio
Key result
Remarks on result:
other: D50= 3,5:1

Applicant's summary and conclusion

Conclusions:
The particles have a median aspect ratio of 3,5:1.
The material is assigned to the “elongated” shape category. The shape is “rod”.