Bis(2-(2-butoxyethoxy)ethyl) adipate

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

28 January 1998 to 30 January 1998

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Remarks:

The reliability score for the study was adopted from the secondary source (US EPA HPV Program). Tests methods were stated to be based on OECD and U.S. EPA guidelines and to follow the appropriate GLP requirements for these. No characterization of the test material was provided. Test concentrations (water accommodated fractions) were nominal.

Qualifier:

equivalent or similar to guideline

Guideline:

EPA OTS 797.1300 (Aquatic Invertebrate Acute Toxicity Test, Freshwater Daphnids)

Deviations:

no

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

Range-finding testA total of 5 water accommodated fractions (WAF) of the test article were prepared in dilution water at 0, 10, 50, 100, 500 and 1000 mg/L using a single replicate at each concentration. The media was not renewed during this test. Definitive test A 1,000 mg/L WAF was prepared by combining 1.5 g of the test substance and 1,500 mL of dilution water in a vessel with a magnetic stirrer. The mixture was stirred for 24 hours and allowed to settle for approximately 1 hour. The water phase containing the WAF was removed by syphon. This was used to formulate definitive concentrations of 0, 130, 220, 360, 600 and 1000 mg/L.

Test organisms (species):

Daphnia magna

Details on test organisms:

Juvenile daphnids less than 24-hours old were produced from an in-house culture. The original culture was procured from Aquatic BioSystems Inc.,Fort Collins, Colorado on August 29, 1997.Prior to testing, the daphnid culture was maintained in dilution water under static conditions in a 3.8 liter glass jar. Cultures were supplied withalga, Selenastrum capricornutum, and a yeast/trout chow mixture daily throughout the acclimation period. No mortality was observed in the 48 h immediately preceding the start of the toxicity test. The average wet weight of control daphnids at the end of the toxicity test was 0.45 mg. The loading was 0.18 g/L.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Post exposure observation period:

none

Hardness:

Stock dilution water was adjusted to a hardness of 160 to 180 mg/L as CaCO3.

Test temperature:

20 +/- 1 °C

pH:

7.7

Dissolved oxygen:

mean 8.7 mg/L (range = 8.5 to 8.8 mg/L)

Salinity:

not measured

Nominal and measured concentrations:

Range-finding test: 0, 10, 50, 100, 500 and 1000 mg/L (WAF)
Definitive test: 0, 130, 220, 360, 600 and 1000 mg/L (WAF)

Details on test conditions:

TEST SYSTEM
- Test vessel: 300 mL
- Type: open
- Material, size, headspace, fill volume: glass, containing 250 mL, water depth approx. 9 cm
- Aeration: no
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
- Biomass loading rate: 40 daphnids/liter

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: deionized tap water, Marblehead, MA
- Total organic carbon: 1.1 mg/L
- Particulate matter: not reported
- Metals: calcium, 24.9 mg/L; magnesium, 22.6 mg/L; sodium, 47.0 mg/L
- Pesticides: none detected
- Chlorine: 3.9 mg/L
- Alkalinity: 76 mg/L (definitive test)
- Ca/mg ratio: 1.1
- Conductivity: mean 590 micromhos/cm (definitive test)
- Culture medium different from test medium: no
- Intervals of water quality measurement: daily

OTHER TEST CONDITIONS
- Adjustment of pH: dilution water adjusted to pH of <8 with phosphoric acid
- Photoperiod: 16 hour light / 8 hour dark (15 minute transition period)
- Light intensity: fluorescent, 4 microEin/m²-sec

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

680 mg/L

Nominal / measured:

nominal

Conc. based on:

other: test mat. (WAF)

Basis for effect:

mobility

Remarks on result:

other: 95% interval: 600 to 790 mg/L

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

420 mg/L

Nominal / measured:

nominal

Conc. based on:

other: test mat. (WAF)

Basis for effect:

mobility

Remarks on result:

other: 95% interval: 600 to 790 mg/L

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

220 mg/L

Nominal / measured:

nominal

Conc. based on:

other: test mat. (WAF)

Basis for effect:

mobility

Details on results:

In the range-finding test, there was complete survival of daphnids at the 0, 10, 50 and 100 mg/L concentrations and complete mortality at higher concentrations.Refer to Table 1 for the result from the definitive study.

Table 1: Survival and sublethal effects
Nominal Concentration (mg/L, WAF)	Live Daphnids (affected Daphnids)
	Replicate	0 hr	24 hr	48 hr
0 (control)	1	10	10	10
	2	10	10	10
130	1	10	10	10
	2	10	10	10
220	1	10	10	10
	2	10	10	10
360	1	10	10	10 (3*)
	2	10	10	10 (1*)
600	1	10	8 (1*)	0
	2	10	7 (1*)	0
1,000	1	10	7 (5*)	0
	2	10	1 (1*)	0
* - affected daphnids were immobilized

Table 2: Validity criteria for OECD 202.

Criterion from the guideline	Outcome	Validity criterion fulfilled
In the control, including the control containing the solubilising agent, not more than 10% of the daphnids should have been immobilized.	0%	yes
The dissolved oxygen concentration at the end of the test should be ≥ 3 mg/L in control and test vessels.	8.5 to 8.8 mg/L	yes

 

Validity criteria fulfilled:

yes

Remarks:

See "Any other information on results incl. tables"

Executive summary:

The 24 -hour EC50 value of the test material was 680 mg/L. The 48 -hour EC50 value was 420 mg/L. The 48 -hour NOEC was 220 mg/L. All concentrations were expressed as the WAF.

Description of key information

EC50 (48 h): 420 mg/L (nominal) for Daphnia magna (similar to OECD 202)

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

420 mg/L

Additional information

One study is available on the short-term toxicity of Bis(2-(2-butoxyethoxy)ethyl) adipate (CAS 141-17-3) to aquatic invertebrates.

This key study was performed according to a procedure similar to OECD Guideline 202. The test organism Daphnia magna was exposed to nominal concentrations of 0, 130, 220, 360, 600 and 1000 mg/L for 96 hours. An EC50 (48 h) of 420 mg/L was calculated.