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Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
genetic toxicity in vitro, other
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)

Data source

Reference
Reference Type:
publication
Title:
Genotoxicity of selected metal compounds in the SOS chromotest
Author:
Lantzsch, H. and Gebel, T.
Year:
1997
Bibliographic source:
Mut.Res. 389(2-3):191-197
Report Date:
1996

Materials and methods

Principles of method if other than guideline:
SOS chromotest was performed as described by Quillardet and Hofnung (1985) with modifications described in Mersch-Sundermann et al. (1991,1993)
GLP compliance:
not specified
Type of assay:
other: SOS chromotest

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
not specified
Specific details on test material used for the study:
Tested metal compounds: SbCl3
SbCl3 concentration: 11-707 µM
SOS chromotest: rapid bacterial genotoxicity test
Tester strain Escherichia coli PQ37 (possess LacZ gene fused to sfiA SOS operon)

Method

Target gene:
LacZ gene
Species / strain
Species / strain / cell type:
E. coli, other: PQ37
Test concentrations with justification for top dose:
SbCl3 concentration: 11-707 µM
Vehicle / solvent:
DMSO
Controls
Untreated negative controls:
yes
Negative solvent / vehicle controls:
yes
True negative controls:
yes
Positive controls:
yes
Details on test system and experimental conditions:
100 ml of a 13-h overnight culture of E. coli PQ37 was diluted with 5.1 ml LA-medium (L- medium + ampicillin) and incubated until a trans-mission of 40% at 600 nm was reached corresponding to 200=106 CFUrml.. This culture was diluted 1:40 with L-medium resulting in 5*10^6 CFU/mL. Test substances, dissolved in 20 ml distilled water or, if not dissolvable in H2O, in DMSO, were given to 600 ml portions of this bacterial nutrient dilution, corresponding 3+*10^3 CFU/test tube. 20 µl of DMSO or distilled water were taken as negative control and the highest substance concentration was used as reagent blank. If the dissolved test compounds were of intensive color with a maximum of absorption near 405 nm, a blank was taken of each concentration and this value substracted from the Bg- and ap-absorption measurements in order to avoid false-negative results.

Results and discussion

Test results
Species / strain:
E. coli, other: PQ37
Genotoxicity:
negative

Applicant's summary and conclusion

Conclusions:
SbCl3 was unable to induce a significant SOS response what indicates no genotoxicity.
Executive summary:

One of the aims of the study was to investigate in vitro genotoxicity of SbCl3 through indicator assay SOS chromotest. Indicator assays suggest genotoxic activity of SbCl3. Mutation assay in in vivo SOS chromotest made on E.coli gave negative response. This result can suggest no genotoxicity. On the other hand, as the test only suggest genotoxic activity, but do not themselves provide direct evidence of mutagenicity, can be considered as unreliable when applied to metals and to antimony in particular.