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EC number: 202-764-2 | CAS number: 99-54-7
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- other: Documentation insufficient. Only raw data available. Apparently problems with emulsifying 1,2-dichloro-4-nitrobenzene
- Principles of method if other than guideline:
- closed bottle test
- GLP compliance:
- no
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- other: activated sludge, adapted and non-adapted
- Details on inoculum:
- adaption not specified
- Duration of test (contact time):
- 20 d
- Parameter:
- other: BOD
- Value:
- 0
- Sampling time:
- 20 d
- Details on results:
- Degradation products: not measured
- Interpretation of results:
- under test conditions no biodegradation observed
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- other: Documentation insufficient. only raw data available. Apparently problems with emulsifying 1,2-dichloro-4-nitrobenzene
- Principles of method if other than guideline:
- closed bottle test
- GLP compliance:
- no
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- other: activated sludge, adapted and non-adapted
- Details on inoculum:
- adaption not specified
- Duration of test (contact time):
- 20 d
- Parameter:
- other: BOD
- Value:
- 0
- Sampling time:
- 20 d
- Details on results:
- Degradation products: not measured
- Interpretation of results:
- under test conditions no biodegradation observed
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- other: Documentation insufficient for assessment.
- Principles of method if other than guideline:
- Method: other: respirometry test
- GLP compliance:
- no
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, non-adapted
- Initial conc.:
- 90 mg/L
- Based on:
- test mat.
- Parameter:
- not specified
- Value:
- < 10
- Sampling time:
- 21 d
- Details on results:
- DOC = 35 mg/l saturated solution
COD = 75 mg O2/l saturated solution
BSB(5) = <10 mg O2/l saturated solution
TS concentration = ca. 90 mg/l
Kinetic of test substance (in %):
< 10 after 5 day(s)
< 10 after 10 day(s)
< 10 after 15 day(s)
Degradation products: not measured - Interpretation of results:
- under test conditions no biodegradation observed
Referenceopen allclose all
Emulsifier was not effective in dissolving 1,2-dichloro-4-nitrobenzene at 1000 mg/l (which equals 958 mg/l COD). The solution contained only 28 % of the theoretical COD (= 270 mg/l).
The solution had a pH of 4.7; it is not indicated whether the solution was neutralized.
Although it was shown in 1982 (Bayer 1982), that 2 g/l emulsifier were not effective in dissolving 1,2-dichloro-4-nitrobenzene at 1000 mg/l (which equals 958 mg/l COD), this part of the experiment was repeated with 1 g/l of emulsifier. The solution contained only 26 % of the theoretical COD (= 253 mg/l).
Only short summary available.
Description of key information
Based on the results of several tests there is evidence that 1,2-dichloro-4-nitrobenzene is not readily biodegradable (Bayer 1983, Bayer 1984; Hoechst 1982).
For non-adapted microorganisms biodegradation of the test item is not expected.
Key value for chemical safety assessment
- Biodegradation in water:
- not biodegradable
- Type of water:
- freshwater
Additional information
All available tests have several drawbacks as the documentation is insufficient for assessment (Bayer 1983, Bayer 1984, Hoechst 1982). But they all have in common that they showed the lack of ready biodegradability of 1,2-dichloro-4-nitrobenzene.
Therefore, the studies can be part of a Weight-of-Evidence approach supporting the evidence that 1,2 -dichloro-4 -nitrobenzene is not readily biodegradable as this is the most stringent classification with respect to environmental hazards.
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