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Diss Factsheets

Toxicological information

Acute Toxicity: dermal

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Administrative data

Endpoint:
acute toxicity: dermal
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
1975
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Study well documented, meets generally accepted scientific priciples, only female rats were used

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1975
Report date:
1975

Materials and methods

Principles of method if other than guideline:
TS (40 % solution) was applied on the clipped skin of the back under occlusive conditions over 24 hrs, then the skin was washed,
post application observation period: 14 d
feed and water ad libitum
observation for signs of intoxication
rats were weighed daily (excluding weekend)
after termination of the observation time: rats were necropsied and gross pathologically examined
GLP compliance:
no
Test type:
standard acute method
Limit test:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
1,2-dichloro-4-nitrobenzene
EC Number:
202-764-2
EC Name:
1,2-dichloro-4-nitrobenzene
Cas Number:
99-54-7
Molecular formula:
C6H3Cl2NO2
IUPAC Name:
1,2-dichloro-4-nitrobenzene
Details on test material:
no data on purity

Test animals

Species:
rat
Strain:
Wistar
Sex:
female
Details on test animals or test system and environmental conditions:
TEST ANIMAL
- Weight at study initiation: 167-193 g
- Housing: individually
- Diet . ad libitum
- Water: ad libitum

Administration / exposure

Type of coverage:
occlusive
Vehicle:
other: sesame oil
Details on dermal exposure:
TEST SITE
- Area of exposure: 30 cm²
- Type of wrap if used: alu foil

Duration of exposure:
24 hours
Doses:
2000 mg/kg bw
No. of animals per sex per dose:
6
Control animals:
no
Details on study design:
- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: daily
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight, gross pathological examination
Statistics:
no

Results and discussion

Effect levels
Sex:
female
Dose descriptor:
LD50
Effect level:
> 2 000 mg/kg bw
Based on:
test mat.
Mortality:
No mortality observed: 0/6
Clinical signs:
other:
Gross pathology:
The macroscopic examination after 14 d showed no adverse effects.

Applicant's summary and conclusion

Interpretation of results:
GHS criteria not met
Conclusions:
An LD50 > 2000 mg/kg bw is determined.
Executive summary:

Single dermal application of 1,2 -Dichloro-4-nitrobenzene at a dose of 2000 mg/kg bw/d to the shaved, intact back of 6 female rats caused no mortality and no clinical findings. Thus the LD50 > 2000 mg/kg bw is determined (Hoechst AG 1975).