1,4-Diazabicyclo[2.2.2]octane-2-methanol

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

24 - 28 Feb 2014

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

yes

Analytical monitoring:

yes

Details on sampling:

Details on sampling
- Concentrations: Water samples were taken for quantitative analysis at the start of the exposure, before and after renewal at 48 hours and the end of the exposure (one sample was analyzed in duplicate, mean value was determined).

Vehicle:

no

Details on test solutions:

- Method: 0.4 g of test substance (adapted to purity) was dissolved in 4 L of test solution. One test vessels (5 L glass beaker covered with plastic lid) were prepared for each test concentration group.
- Eluate: no
- Differential loading: yes
- Controls: yes, test medium control

Test organisms (species):

Oryzias latipes

Details on test organisms:

TEST ORGANISM
- Common name: Ricefish
- Source: RIVER SKETCH
- Age at study initiation (mean): less than 1 year
- Length at study initiation (mean and range): 2.6 cm (2.0 - 2.8 cm)
- Weight at study initiation (mean and range): 0.17 g (0.07 - 0.21 g)
- Feeding during test: none

ACCLIMATION
- Acclimation period: 62 days (after rearing for 15 days in a rearing tank under flow-through system)
- Acclimation conditions: In a static water chamber (with circulating water cleaning filtering system), same as test
- Type and amount of food: Tetrafin (formula feed)
- Feeding frequency: Daily
- Health during acclimation (any mortality observed): During 7 days before testing, mortality or disease rate was < 5%

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Test temperature:

21.3 - 22.0 °C

pH:

7.54 - 8.93

Dissolved oxygen:

6.2 - 8.7 mg/L

Nominal and measured concentrations:

nominal: control, 100 mg/L
measured: < LOD, 94.5 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 5 L glass beaker, covered with plastic lid
- Fill volume: 4 L/vessel
- Renewal rate of test solution: 48 hours
- No. of organisms per vessel: 7 fish/test group
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 0.3 g/L fish density

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Tap water was filtered through a PF filter, thereafter through an activated charcoal filter and again through a PF filter. Measurement values (like hardness, particulate matter, pH-value, total organic carbon, etc.) were confirmed within the range of values according to 'Water Quality Criteria for Fisheries' (Japan Fisheries Resource Conservation Association, March, 2006), OECD 210 and Health and Welfare Ministry ordinance No. 101, January 28 2011
- Intervals of water quality measurement: More than once every six month

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h light / 8 h dark (room light)

TEST CONCENTRATIONS
- Range finding study : yes
- Test concentrations: nominal test concentrations of 10 and 100 mg/L
- Results used to determine the conditions for the definitive study: No mortality or abnormal behaviour was observed in both concentration levels.

Reference substance (positive control):

yes

Remarks:

Copper (II) sulfate pentahydrate

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 94.5 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- Behavioural abnormalities: No abnormal behaviour in control and test concentration.
- Mortality of control: none

Results with reference substance (positive control):

The result of the test with the standard substance (performed in January, 2014) was within the normal range in this laboratory (96 h LC50 = 1.8 mg/L, 95% CI: 1.2 - 2.8 mg/L)

Sublethal observations / clinical signs:

The dissolved oxygen concentration during the exposure period was maintained more than 60% of saturated concentrations.

Table 1: Mortality of Oryzias latipes

Nominal (measured) concentration (mg/L)	Incubation time [h]
	0	6	24	48	72	96
Control	0	0	0	0	0	0
100 (94.5)	0	0	0	0	0	0

 

Validity criteria fulfilled:

yes

Description of key information

LC50 (96 h): > 100 mg/L (nominal) for Oryzias latipes (OECD 203)
LC50 (96 h): > 94.5 mg/L (measured) for Oryzias latipes (OECD 203)

Additional information

One experimental study investigating the short-term effects of the test substance to fish is available (Tosoh Corporation, 2014). The study was performed according to OECD 203 under GLP conditions with the ricefish Oryzias latipes.In this limit test under semi-static conditions no mortality was observed after 96 h at the nominal concentration of 100 mg/L (corresponding to a mean measured value of 94.5 mg/L).