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Environmental fate & pathways

Biodegradation in water: screening tests

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Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1997
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: see 'Remark'
Remarks:
The reliability rating is a 1 because the study followed an OECD standard guideline, which describes a procedure designed to evaluate this endpoint, and the results were reviewed for reliability and assessed as valid. The study was also conducted under GLP.

Data source

Reference
Reference Type:
other company data
Title:
Unnamed
Year:
1997

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
GLP compliance:
yes

Test material

Constituent 1
Reference substance name:
Alkenes, C6-10, hydroformylation products, high-boiling
EC Number:
271-230-9
EC Name:
Alkenes, C6-10, hydroformylation products, high-boiling
Cas Number:
68526-82-9
Molecular formula:
None available
IUPAC Name:
None available - not a single isomer - see remarks
Constituent 2
Reference substance name:
Mixed HOF
IUPAC Name:
Mixed HOF

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic, non-adapted
Details on inoculum:
Inoculum used as the source of the microorganisms was an aliquot of fresh activated sludge from a domestic wastewater treatment facility in New Jersey, USA.
Duration of test (contact time):
28 d
Initial test substance concentration
Initial conc.:
ca. 44 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
O2 consumption
Reference substance
Reference substance:
benzoic acid, sodium salt

Results and discussion

% Degradation
Parameter:
% degradation (O2 consumption)
Value:
49.99
Sampling time:
28 d
Remarks on result:
other: Based on ThOD
Details on results:
The approximate half-life was reached by day 28, at which time 49.99% degradation of the test material was observed.

By day 14, >60% biodegradation of positive control was observed, which met the guideline requirement. No excursions from the protocol were noted.
Biodegradation was based on oxygen consumption and the theoretical oxygen demand of the test material as calculated using results of an elemental analysis of the test material.

% Degradation* Mean % Degradation
Sample (day 28) (day 28)
Test Material 52.8, 52.1, 45.1 49.99
Na Benzoate 87.2, 88.5 88.1

* replicate data

BOD5 / COD results

Results with reference substance:
Sodium benzoate biodegraded 68.1% after 3 days and 88.1% on day 28.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Interpretation of results:
inherently biodegradable
Conclusions:
Mixed HOF reached 49.99% biodegradation in 28 d.
Executive summary:

Mixed HOF reached 49.99% biodegradation in 28 d.