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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Link to relevant study record(s)

Description of key information

No effects on acute toxicity to aquatic invertebrates up to the limit of water solubility.

Key value for chemical safety assessment

Additional information

In general, all EC/EL50(48h) values derived with different invertebate species for various test substances in the SCAE C2 -C8 category were above the limit of water solubility, so that no toxicity of dissolved substance was found (chemical toxicity). There were, however, several descriptions of indirect toxicity by physical effects of undissolved test substance at concentrations between 1 and 100 mg/L.

Several studies on toxicity to aquatic invertebrates were available for Fatty acids, C8-16, 2-ethylhexyl esters.

The key study was performed according to the EU-method C.2 and to GLP under static conditions (Wierich 1998). Different preparation methods of test solution with dilution water were applied and tested on Daphnia magna. Effects on mobility of the daphnids (about 50 %) were reported for the medium which contained 100 mg/L test substance without further separation of the undissolved test substance (measured concentration at the start of the test: 12.41 mg/L). After filtration of the test solution no effect on mobility was stated (measured concentration at the start of the test: 0.04 mg/L). Therefore, it can be stated, that the observed effects are most likely physical and not systemic effects. The resulting EC50(48h) was reported as >100 mg/L (nominal).

In a supporting study (Scholz 1995), which was also performed according to EU method C.2 under GLP-conditions, the effect of the test substance was tested on Daphnia magna using a WAF (preparation of test solution via centrifugation). An immobility of 18 and 30 % after 48 h was stated in the 3 and 100 mg/L test concentration (nominal, WAF), respectively. The measured concentrations were in a range of 0.09 - 0.3 mg/L, the water solubility of the test substance was determined as < 0.05 - 0.3 mg/L. So the analytical recovery of the test substance was in the upper part of the water solubility range. The authors stated, that it can not be excluded that the test solutions were partly/temporarily supersaturated. Therefore the observed effects could be due to physical effects of the test substance and an EL50(48h) > 100 mg/L (nominal loading rate) was given.

A supporting study on toxicity to the marine species Acartia tonsa with Fatty acids, C8-16, 2-ethylhexyl esters (tested using a WAF) reported an EL50(48h) >2000 mg/L (Gibson 1999).