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Ecotoxicological information

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Based on a review of the ecotoxicity values from the acute fish, daphnid, and algal studies, the key studies indicated that EDHO exhibited acute toxicity (LC/EC50) values ranging from 1.08 to 244 mg/L.  Freshwater green algae were the most sensitive of the three trophic levels to EDHO. The 72-hour ErC50 based on the growth rate endpoint was 1.08 mg/L and the NOEC was 0.513 mg /L.

A chronic 21-day reproduction test with Daphnia magna determined a NOEC of 5 mg/L.

EDHO is rapidly hydrolyzed to AEPD and formaldehyde, with half-lives of less than 4 hours at pH 4, 7, and 9 at 25 ºC. As a result of rapid hydrolysis and low potential for continuous input in the aquatic environment based on use patterns, potential for chronic exposure to EDHO is expected to be low. Bioaccumulation potential is expected to be minimal based on a reported log Kow of -0.32.


In addition, acute testing clearly indicated that fish were the least sensitive of the three trophic levels to EDHO exposure. The fish acute values ranged from 130 to 244 mg/L while the aquatic invertebrate acute values ranged from 16.9 to 138 mg/L and the algal acute values ranged from 1.08 to 2.09 mg/L. Chronic values were determined for algal species and the most sensitive invertebrate (Daphnia magna). The NOEC for the daphnid chronic study was determined to be 5 mg/L, an order of magnitude greater than the most sensitive trophic level (ErC50 for freshwater green alga of 0.513 mg/L. Since fish are vertebrates as well as the least acutely sensitive trophic level, it was neither scientifically nor ethically justified to determine a chronic value. 


According to REACH Annex IX requirements, long-term toxicity testing shall only be considered when the chemical safety assessment indicates the need for further investigations. Hence, long-term testing can be waived for EDHO.