7a-ethyldihydro-1H,3H,5H-oxazolo[3,4-c]oxazole

Specific investigations: other studies

Administrative data

Endpoint:

specific investigations: other studies

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: (meets generally accepted scientific standards, well-documented, and acceptable for assessment)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1982

Materials and methods

Test guideline

Qualifier:

no guideline available

Principles of method if other than guideline:

Endpoint: mortality
LD50

GLP compliance:

no

Type of method:

in vivo

Test material

Test material information

Constituent 1

Reference substance name:

7a-ethyldihydro-1H,3H,5H-oxazolo[3,4-c]oxazole

EC Number:

231-810-4

EC Name:

7a-ethyldihydro-1H,3H,5H-oxazolo[3,4-c]oxazole

Cas Number:

7747-35-5

Molecular formula:

C7H13NO2

IUPAC Name:

7a-ethyl-tetrahydro-1H-[1,3]oxazolo[3,4-c][1,3]oxazole

Test animals

Species:

other: Colinnus virginianus (avian)

Sex:

not specified

Details on test animals or test system and environmental conditions:

Young adult (14-18 week-old) quail were reared in a private colony at the testing facility. Birds were observed for 24 days prior to placement on test, and were examined by a laboratory veterinarian

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

water

Analytical verification of doses or concentrations:

not specified

Duration of treatment / exposure:

Eposure period: 1 minute

Doses / concentrations

Remarks:

Doses / Concentrations:
0, 316, 681, 1000, 1470, and 2150 mg/kg
Basis:

No. of animals per sex per dose:

Birds were randomly divided into 1 vehicle control group (tap water) and 5 treatment groups (5 males and 5 females per group) based on body weights.

Control animals:

yes, concurrent vehicle

Details on study design:

Birds were fasted for 16 hours prior to dosing by gavage, and were permitted a commercial food and water ad libitum at all other times. Birds were housed 10 per pen in an indoor environment with controlled lighting, temperature, and relative humidity. Food consumption was recorded on days 3, 7, 14, and 21. Observations were made daily for clinical signs of toxicity indicative of test material effect. All birds dying during the study and four arbitrarily-selected birds were sacrificed on day 21 and subjected to a complete gross pathological examination.

Examinations

Examinations:

Body weights
Food consumption

Results and discussion

Details on results:

Birds were anorexic and lethargic at doses >681 mg/kg/day. One control bird was cannabalized.

Treated birds found dead had discolored kidneys and liver; survivors were largely normal. Treated birds lost weight and consumed less feed.

The estimated oral LD50 for the test material in quail was 1100 (830-1450) mg/kg bw.

Applicant's summary and conclusion

Conclusions:

The estimated oral LD50 for the test material in quail was 1100 (830-1450) mg/kg.