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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP-Guideline study incl. analytics

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2011
Report date:
2011

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
m-terphenyl-2'-ol
EC Number:
219-401-9
EC Name:
m-terphenyl-2'-ol
Cas Number:
2432-11-3
Molecular formula:
C18H14O
IUPAC Name:
1,1':3',1''-terphenyl-2'-ol
Details on test material:
- Name of test material (as cited in study report): 2,6 Diphenylphenol
- Physical state/Appearance: solid/beige
- Analytical purity: 99.5 %
- Water solubility: 0.51 + 0.01 mg/L at 20°C
- Lot/batch No.: LV 83/2010
- Expiration date of the lot/batch: Feb 2014
- Stability under test conditions: The stability of the test substance as a solution in test media and under testing conditions was determined by concentration control analysis
- Storage condition of test material: The test substance was stored at ambient temperature

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
- Concentrations: 0 (control), 0.22, 0.46, 1.0, 2.2, 4.6, 10, 22 mg/L as nominal concentrations based on test substance mass (loading ratio) without correction for purity
- Sampling method: At the start of the exposure (0 h) samples from vessels without daphnids and at the end of the exposure (48 h) samples from vessels with daphnids were analyzed.
- Sample storage conditions before analysis: Ambient temperature. The samples were transported to the Analytical Laboratory on the day of sampling.

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)

Each test solution was prepared separately (differential loading) by directly adding test substance to test medium. For each concentration, the test substance was weighed into a 1 L or 5 L volumetric flask and filled to the mark with test medium. The mixture was then placed in an ultrasonic bath for 10 minutes and stirred for about 4 days at 20 ± 2 °C. Undissolved test substance was removed by centrifugation (approximately 20 min at about 17700 G, Beckman Coulter TM, Avanti J 25). After centrifugation all test solutions were visibly clear.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Daphnia magna STRAUS
- Source: The clone of Daphnia magna STRAUS 1820 used was supplied by the Institut National de Recherche Chimique Appliquée, France, in 1978.
From this date on this clone was cultured and bred continuously in the Ecotoxicology Laboratory of Experimental Toxicology and Ecology, BASF SE Ludwigshafen Germany.
- Age at study initiation (mean and range, SD): < 24 hours (at least 3rd brood progeny)
- Feeding during test: No feeding during the exposure period.

ACCLIMATION

- Type and amount of food: Unicellular algae (Desmodesmus subspicatus), cultured continuously at the test facility
- Acclimation conditions (same as test or not): The Daphnia are cultured under the identical conditions as the test including test media (Elendt M4), water quality, and temperature (20 ±1°C).
- Feeding: No feeding during the exposure period.

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Hardness:
2.61 mmol/L
Test temperature:
19 °C (continuous monitoring)
pH:
7.6-8.0
Dissolved oxygen:
7.8-8.7
Nominal and measured concentrations:
Nominal: 0.22, 0.46, 1.0, 2.2, 4.6, 10, 22 mg/L
Mean measured concentration: 0.10, 0.10, 0.10, 0.13, 0.14, 0.17, 0.18, mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: glass beakers (nominal volume 100 mL)
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: 50 mL
- Aeration: none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 5 animals / test vessel (0.1 animals/mL)


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to guideline OECD 202
- Total hardness: 2.20-3.20
- Ca/mg ratio: about 4 : 1
- Conductivity: 550 - 650 μS/cm
- pH value: 7.5-8.5


OTHER TEST CONDITIONS
- Light intensity/Photoperiod: About 136 - 614 lux at a wave length of 400 -750 nm; 16 hours light : 8 hours darkness


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Each test vessel was visually checked for immobilized daphnids after 0, 24 and 48 hours. In addition any abnormal behavior or appearance was documented. Throughout the test, the appearance of the test solutions and dissolution behavior of the test substance was observed and recorded daily. The chemical and physical parameters of the test medium (total hardness, acid capacity, pH and conductivity) were determined after aeration and prior to use in the test. Dissolved oxygen, pH and temperature were measured at the start of the exposure and at the end of the exposure in replicate 1 of all test concentrations and the control. In addition, temperature was measured continuously during the whole exposure period in a separate vessel filled with water proximal to the test vessels.

TEST CONCENTRATIONS
- Range finding study: In a preliminary range finding test (experimental conduct in accordance with GLP, without a GLP status) the 48 hour EC50 was between 1 and 10 mg/L. The range of no effect to 100% immobilization was 1 to 100 mg/L. The raw data of the range finding test will be archived together with the raw data of this study.


Reference substance (positive control):
yes
Remarks:
potassium dichromate (K2Cr2O7)

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
4.6 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
8.48 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
> 22 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
0.14 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
0.159 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
> 0.18 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Results with reference substance (positive control):
Tests with a reference substance, potassium dichromate (K2Cr2O7), are conducted monthly. Reference substance tests are conducted according to OECD 202 guidelines and in accordance with GLP, but without a GLP status. The EC50(24h) of the reference substance potassium dichromate was 1.18 mg/L (experiment date: 22 Feb 2011, project number: 50E0063/043039).
Reported statistics and error estimates:
For the statistical evaluation of the data and calculation of the EC50 the probit method (Finney, 1971) was used and performed with the commercial software "TOXRAT Professional 2.10” (ToxRat Solutions GmbH, Alsdorf, Germany).

Any other information on results incl. tables

Conclusion

In conclusion the 48 hour EC50 values for 2,6 Diphenylphenol in this acute toxicity study with Daphnia magna were 8.48 mg/L based on the nominal concentration (loading ratio) of the test substance and 0.159 mg/L based on the mean measured concentrations.

Since the measured concentrations deviated markedly from the nominal concentrations, the effect concentration, which is based on the mean measured concentrations should be preferably used for the evaluation of the test substance.

The results in this study are consistent with all validity criteria and the test is valid according to the guidelines of this study. No deviations from test guidelines or other incidents occurred during the course of the reported test which may have influenced the results.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes