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Administrative data

Endpoint:
in vitro gene mutation study in bacteria
Remarks:
Type of genotoxicity: gene mutation
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: basic information given

Data source

Reference
Reference Type:
other: Summary of Results
Title:
Unnamed
Year:
2010
Report date:
2010

Materials and methods

Principles of method if other than guideline:
no guideline available; modified version of the Ames test (OECD 471)
GLP compliance:
no
Type of assay:
bacterial reverse mutation assay

Test material

Constituent 1
Chemical structure
Reference substance name:
m-terphenyl-2'-ol
EC Number:
219-401-9
EC Name:
m-terphenyl-2'-ol
Cas Number:
2432-11-3
Molecular formula:
C18H14O
IUPAC Name:
1,1':3',1''-terphenyl-2'-ol
Details on test material:
- Name of the test substance used in the study report: 2,6-Diiphenylphenol
-Test substance No.: 10/0493-1
-Batch No.: LV 83/2010
- Purity: 100%
- Date of production: 18 Aug 2010
- Physical state: solid, beige
- Storage conditions: room temperature

Method

Target gene:
histidin operon
Species / strainopen allclose all
Species / strain / cell type:
S. typhimurium TA 98
Species / strain / cell type:
other: S. typhimurium TA Mix (TA 7001 - TA 7006)
Metabolic activation:
with and without
Metabolic activation system:
rat liver S9 fraction
Test concentrations with justification for top dose:
0, 4, 20, 100, 500, 2500 and 5000 µg/ml
Vehicle / solvent:
DMSO
Details on test system and experimental conditions:
Liquid fluctuation test

Results and discussion

Test resultsopen allclose all
Species / strain:
S. typhimurium TA 98
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
no cytotoxicity
Species / strain:
other: S. typhimurium TA Mix (TA 7001 - TA 7006)
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
no cytotoxicity
Additional information on results:
.
Remarks on result:
other: all strains/cell types tested
Remarks:
Migrated from field 'Test system'.

Any other information on results incl. tables

Precipitation in plates:

- at 500 µg/m and above (without S9 mix)

- no precipitation occurred (with S9 mix)

A relevant increase in the number of positive wells (his+ revertants) was not observed either without S9 mix or after the addition of a metabolizing system.

Applicant's summary and conclusion