2,4-dichloro-1,3-dinitro-5-(trifluoromethyl)benzene

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1998

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1998

Report date:

1998

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

2,4-dichloro-1,3-dinitro-5-(trifluoromethyl)benzene

EC Number:

249-420-8

EC Name:

2,4-dichloro-1,3-dinitro-5-(trifluoromethyl)benzene

Cas Number:

29091-09-6

Molecular formula:

C7HCl2F3N2O4

IUPAC Name:

2,4-dichloro-1,3-dinitro-5-(trifluoromethyl)benzene

Details on test material:

Identification: 2,4 dichloro 3,5 dinitro benzotrifluoride
Purity: 97.70%
Stability in water: Maximum 7 days at room temperature
Solubility in water: Insoluble
pH in aqueous solution: 6.44 at a concentration of 100g/L
Storage conditions: at room temperature at about 20°C, light protected

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Daphnia magna

Study design

Test type:

static

Water media type:

saltwater

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Hardness:

250 mg/l

Test temperature:

20-21 °C

Salinity:

CaCl2 x 2H2O : 294 mg/l
MgSO4 x 7H2O : 123 mg/l
NaHCO3 :65 mg/l
KCl: 5.8 mg/l
Alkalinity: 0.8 mmol/l
Ratio of Ca:Mg = 4:1 (based on molarity)
Na:K = 10:1 (based on molarity)

Nominal and measured concentrations:

0.06, 0.13, 0.30, 0.56 and 1.29 mg/l at the start of the test. The value found at the end of the test ranged from 96-100 % of the initially measured values.

Details on test conditions:

The test item was found sufficiently stable during the test period.

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

0.2 mg/L

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 0.16-0.22

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

0.06 mg/L

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

0.06 mg/L

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 4

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

0.56 mg/L

Conc. based on:

test mat.

Basis for effect:

mobility

Any other information on results incl. tables

The mean analytically determined test item concentrations in the analyzed test media samples amounted to 0.06, 0.13, 0.30, 0.56 and 1.29 mg/L at the start of the test. The values found at the end of the test ranged from 96-100% of the initially measured values. Under the test conditions the test item was sufficiently stable during the test period of 48 hours.

All biological results are related to the total mean measured test item concentrations of 0.06mg/L (dilution 1:222), 0.13mg/L (dilution 1:100), 0.29mg/L (dilution 1:45.5), 0.56mg/L (dilution 1:22.2) and 1.27mg/L (dilution 1:10), calculated as the average over all measurements per test concentration during the test period.

Applicant's summary and conclusion

Conclusions:

The 48-hour EC50 was calculated to be 0.2mg/L with 95% confidence limits from 0.16 to 0.22mg/L.