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EC number: 254-052-6 | CAS number: 38640-62-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
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- Flash point
- Auto flammability
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- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
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- Stability: thermal, sunlight, metals
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- Additional physico-chemical information
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- Endpoint summary
- Stability
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- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
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- Toxicological Summary
- Toxicokinetics, metabolism and distribution
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- Additional toxicological data

Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 986
- Report date:
- 1986
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.13/14 (Mutagenicity - Reverse Mutation Test Using Bacteria)
- Deviations:
- yes
- Remarks:
- : only four strains tested
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Bis(isopropyl)naphthalene
- EC Number:
- 254-052-6
- EC Name:
- Bis(isopropyl)naphthalene
- Cas Number:
- 38640-62-9
- Molecular formula:
- C16H20
- IUPAC Name:
- bis(isopropyl)naphthalene
- Details on test material:
- - Name of test material (as cited in study report): RKKO 131006
- Physical state: colorless liquid
- Analytical purity: no data
- Isomers composition: no data
- Storage condition of test material: at room temperature in the dark
Constituent 1
Method
- Target gene:
- his
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Metabolic activation system:
- S-9 mix from Aroclor induced rat liver (Litton Bionetics Inc.)
- Test concentrations with justification for top dose:
- 0, 0.1, 0.3, 1.0, 3.0, and 10.0 µl/plate (0.092 - 9.2 mg/plate)
- Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: DMSO
- Justification for choice of solvent/vehicle: insolubility of test substance in water
Controls
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: without S-9: Sodium azide (TA100, TA1537), 5-Nitrofluorene (TA98), 9-Aminoacridine (TA1537); with S-9: 2-Aminoanthracene
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in agar (plate incorporation)
DURATION
- Exposure duration: 3 days
- Expression time (cells in growth medium): 3 days at 37° C
DETERMINATION OF CYTOTOXICITY
- Method: relative total growth; toxicity of the test substance was evaluated in a separate test by incubating tester strain TA100 with 10 graduate concentrations of test substance (0 - 200 µL/plate, spacing factor 2). - Evaluation criteria:
- - The solvent/vehicle control has to show an adequate number of revertants.
- A dose-response relationship must be demonstrated at least for one tester strain.
- The maximum number of revertants must be at least double of spontaneous revertants.
- The result must be reproducible. - Statistics:
- no data
Results and discussion
Test results
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity, but tested up to precipitating concentrations
- Remarks:
- no toxicity to TA 100 up to 200 µl/plate
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Additional information on results:
- TEST-SPECIFIC CONFOUNDING FACTORS
- Precipitation: The test substance proved to be insoluble at 12.5 µl and higher, i.e. above recommended maximum test concentration of 5 mg/pl.
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
The test substance did not induce mutant colonies above background in any strain at any concentration tested.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
In a bacterial reverse mutation assay using four strains of S. typhimurium, there was no evidence of an increase in revertant colonies above background in any strain and at any concentration tested with and without metabolic activation. Diisopropylnaphthalene proved to be negative in the Ames test under the test conditions used.
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