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Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
in vitro gene mutation study in bacteria
Remarks:
Type of genotoxicity: gene mutation
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
3 (not reliable)
Rationale for reliability incl. deficiencies:
other: Test method was similar to OECD guideline 471, but there is no data on positive controls. No data on GLP.

Data source

Reference
Reference Type:
secondary source
Title:
Mutagenicity test data of existing chemical substances based on the toxicity investigation system of the industrial safety and health law.
Author:
Japan Chemical Industry Ecology-Toxicology & Information Center (JETOC)
Year:
2005
Bibliographic source:
Japan Chemical Industry Ecology-Toxicology & Information Center (JETOC). Mutagenicity test data of existing chemical substances based on the toxicity investigation system of the industrial safety and health law. Supplement 3, 2005.

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 471 (Bacterial Reverse Mutation Assay)
Deviations:
not specified
Remarks:
(No data on positive controls)
GLP compliance:
not specified
Type of assay:
bacterial reverse mutation assay

Test material

Constituent 1
Chemical structure
Reference substance name:
3-chloropropionic acid
EC Number:
203-534-4
EC Name:
3-chloropropionic acid
Cas Number:
107-94-8
Molecular formula:
C3H5ClO2
IUPAC Name:
3-chloropropanoic acid

Method

Species / strainopen allclose all
Species / strain / cell type:
S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
Species / strain / cell type:
E. coli WP2 uvr A pKM 101
Metabolic activation:
with and without
Metabolic activation system:
Rat, Liver, S9, Sodium phenobarbital and 5,6-benzoflavone (10%)
Test concentrations with justification for top dose:
0, 1.22, 4.88, 19.5, 78.1, 313, 1250, 5000 µg/plate
Vehicle / solvent:
Water
Controls
Negative solvent / vehicle controls:
yes
Positive controls:
not specified
Details on test system and experimental conditions:
Method: Preincubation

Results and discussion

Test resultsopen allclose all
Species / strain:
S. typhimurium, other: TA 100 and TA 1535
Metabolic activation:
with and without
Genotoxicity:
positive
Cytotoxicity / choice of top concentrations:
not specified
Vehicle controls validity:
not specified
Positive controls validity:
not specified
Species / strain:
E. coli WP2 uvr A pKM 101
Metabolic activation:
with and without
Genotoxicity:
positive
Cytotoxicity / choice of top concentrations:
not specified
Vehicle controls validity:
not specified
Positive controls validity:
not specified
Species / strain:
S. typhimurium, other: TA 98 and TA 1537
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
not specified
Vehicle controls validity:
not specified
Positive controls validity:
not specified
Remarks on result:
other: all strains/cell types tested
Remarks:
Migrated from field 'Test system'.

Any other information on results incl. tables

Test substance was mutagenic on Salmonella typhimurium TA 100 and TA 1535, and on E. coli WP2 uvrA pKM 101, with and without metabolic activation. It was non-mutagenic on Salmonella typhimurium TA 98 and TA 1537, with and without metabolic activation.

Applicant's summary and conclusion

Conclusions:
Interpretation of results (migrated information):
ambiguous

Test substance was mutagenic on Salmonella typhimurium TA 100 and TA 1535, and on E. coli WP2 uvrA pKM 101, with and without metabolic activation. It was non-mutagenic on Salmonella typhimurium TA 98 and TA 1537, with and without metabolic activation.
Executive summary:

3-chloropropanoic acid was tested for mutagenicity in the strains TA 100, TA 1535, TA 1537, and TA 98 of Salmonella typhimurium and in E. coli WP2 uvrA pKM 101. The mutagenicity studies were conducted in the absence and in the presence of a metabolizing system derived from rat liver homogenate. The tested concentrations were: 0, 1.22, 4.88, 19.5, 78.1, 313, 1250, and 5000 µg/plate.

Test substance was mutagenic on Salmonella typhimurium TA 100 and TA 1535, and on E. coli WP2 uvr A pKM 101, with and without metabolic activation. It was non-mutagenic on Salmonella typhimurium TA 98 and TA 1537, with and without metabolic activation.